Colon Mucosal Cells after High-Dose Fractional Irradiation

Zorc-Pleskovič, Ruda; Vraspir-Porenta, O.; Petrovič, D.; Zorc, M.; Pleskovič, L.

doi:10.14712/fb2000046010043

Fol. Biol. 2000, 46, 43-48

https://doi.org/10.14712/fb2000046010043

Colon Mucosal Cells after High-Dose Fractional Irradiation

Ruda Zorc-Pleskovič¹, O. Vraspir-Porenta¹, D. Petrovič¹, M. Zorc¹, L. Pleskovič²

¹Institute of Histology and Embryology, Medical Faculty, University of Ljubljana, Ljubljana, Slovenia
²Department of Gastroenterologic Surgery, University Medical Centre of Ljubljana, Ljubljana, Slovenia

Received May 10, 1999
Accepted August 12, 1999

The aim of this study was to investigate histological and stereological changes in cryptal enterocytes, mucosal lymphocytes and mast cells 10 days after irradiation. For experimental model, 24 Beagle dogs 1-2 years old were used. Twelve dogs were irradiated 20 days with 32 Gy over the whole pelvis and tail. Another 12 dogs represented a control group. For the detection of apoptosis, the TUNEL technique was used. Histological and stereological analyses were performed using a Wild sampling microscope M 1000. In the irradiated group, volume density (P < 0.01), numerical density (P < 0.05) and average volume of lymphocytes (P < 0.001) were significantly lower than in the nonirradiated group. Numerical areal density of mast cells in the irradiated group was also significantly lower (P < 0.05). Volume density (P < 0.001) and average volume of mast cells (P < 0.001) were significantly higher in the irradiated group. The results of our experiments show that irradiation causes injury and loss of lymphocytes and mast cells in the colon mucosa. Apoptosis was detected in enterocytes and lymphocytes in the irradiated group and in nonirradiated group in equal numbers (2.5 ± 0.3 vs. 2.3 ± 0.3; ns.), suggesting that 10 days after high-dose irradiation, the cell loss is not due to apoptosis.