Fol. Biol. 2026, 72, 68-74
https://doi.org/10.14712/fb2026.0007
Cytoprotective Effects of Tauroursodeoxycholic Acid in Heat Stress-Induced Endoplasmic Reticulum Stress in Male Germ Cells
References
1. , A., Tanjore, H., Blackwell, T. S. (2018) Endoplasmic reticulum stress in pulmonary fibrosis. Matrix Biol. 68, 355-365.
<https://doi.org/10.1016/j.matbio.2018.03.015>
2. , L., Leites, I., Romăo, R. et al. (2022) Impact of heat stress on bovine sperm quality and competence. Animals (Basel) 12, 975.
<https://doi.org/10.3390/ani12080975>
3. , X., Cubillos-Ruiz, J. R. (2021) Endoplasmic reticulum stress signals in the tumour and its microenvironment. Nat. Rev. Cancer 21, 71-88.
<https://doi.org/10.1038/s41568-020-00312-2>
4. , K., Ali, K., Ghina, G. (2019) The developmental process of spermatogenesis. J. Androl. Gynaecol. 7, 3.
5. , D., Agarwal, A., Ong, C. (2015) Causes, effects and molecular mechanisms of testicular heat stress. Reprod. Biomed. Online 30, 14-27.
<https://doi.org/10.1016/j.rbmo.2014.09.018>
6. , Z., Gao, W., Jiang, Q. et al. (2022) Targeting IRE1α and PERK in the endoplasmic reticulum stress pathway attenuates fatty acid-induced insulin resistance in bovine hepatocytes. J. Dairy Sci. 105, 6895-6908.
<https://doi.org/10.3168/jds.2021-21754>
7. , Y., Wang, C., Wang, K. et al. (2022) The effects and molecular mechanism of heat stress on spermatogenesis and mitigation measures. Syst. Biol. Reprod. Med. 68, 331-347.
<https://doi.org/10.1080/19396368.2022.2074325>
8. , A. M., Healy, S. J., Jäger, R. et al. (2012) Stress management at the ER: regulators of ER stress-induced apoptosis. Pharmacol. Ther. 134, 306-316.
<https://doi.org/10.1016/j.pharmthera.2012.02.003>
9. , K., Wolf, F., Becker, A. et al. (2009) Isolation and cultivation of stem cells from adult mouse testes. Nat. Protoc. 4, 143-154.
<https://doi.org/10.1038/nprot.2008.242>
10. , N. I., Mohamed, A. S., Sheikh Abdul Kadir, S. H. et al. (2018) Overview of bile acids signaling and perspective on ursodeoxycholic acid. Biomolecules 8, 159.
<https://doi.org/10.3390/biom8040159>
11. Henson, R. N. (2015) Analysis of variance (ANOVA). In: Brain Mapping: An Encyclopedic Reference, ed. Toga, A. W., pp. 477-481, Elsevier, Amsterdam.
12. , C. (2012) The unfolded protein response: controlling cell fate decisions under ER stress and beyond. Nat. Rev. Mol. Cell Biol. 13, 89-102.
<https://doi.org/10.1038/nrm3270>
13. , A. P., Dang-Thi, A. T., Phan-Van, S. et al. (2022) Impact of high ambient temperature on human sperm parameters: a meta-analysis. Iranian J. Public Health 51, 710.
14. , P. J., Oosthuizen, M. K., Mitchell, C. et al. (2020) Heat and dehydration-induced oxidative damage following incubator heat stress. PLoS One 15, e0242279.
<https://doi.org/10.1371/journal.pone.0242279>
15. Katikireddy, K. R., O’Sullivan, F. (2011) Immunohistochemical and immunofluorescence procedures for protein analysis. In: Gene Expression Profiling: Methods and Protocols, ed. Lorraine O’Driscoll, pp. 155-167, Humana Press, Totowa.
16. , R., Katirci, E., Onal, T. et al. (2025) The effects of ozone exposure on apoptotic mechanisms in Colo-320 and Colo-741 cell lines. J. Mol. Histol. 56, 136.
<https://doi.org/10.1007/s10735-025-10409-3>
17. , H., Wada, Y., Konno, T. et al. (2020) Endoplasmic reticulum stress attenuation promotes bovine oocyte maturation in vitro. Reproduction 159, 361-370.
<https://doi.org/10.1530/REP-19-0492>
18. , I., Xu, W., Reed, J. C. (2008) Cell death and endoplasmic reticulum stress: disease relevance and therapeutic opportunities. Nat. Rev. Drug Discov. 7, 1013-1030.
<https://doi.org/10.1038/nrd2755>
19. , J. H., Park, S. J., Kim, T. S. et al. (2013) Testicular hyperthermia induces unfolded protein response activation in spermatocytes. Biochem. Biophys. Res. Commun. 434, 861-866.
<https://doi.org/10.1016/j.bbrc.2013.04.032>
20. , M. (2019) Tauroursodeoxycholate – bile acid with chaperoning activity. Cells 8, 1471.
<https://doi.org/10.3390/cells8121471>
21. , Y. Y., Hong, S. H., Lee, Y. J. et al. (2010) Tauroursodeoxycholate enhances islet function by reducing ER stress. Biochem. Biophys. Res. Commun. 397, 735-739.
<https://doi.org/10.1016/j.bbrc.2010.06.022>
22. , Y., Peterson, D. A., Kimura, H. et al. (1997) Mechanism of MTT reduction. J. Neurochem. 69, 581-593.
<https://doi.org/10.1046/j.1471-4159.1997.69020581.x>
23. , Y., Sakamoto, H., Adachi, M. et al. (2012) Heat stress activates ER stress signals in rats. Mol. Biol. Rep. 39, 3987-3993.
<https://doi.org/10.1007/s11033-011-1179-2>
24. , J. D., Kaufman, R. J. (2007) The endoplasmic reticulum and the unfolded protein response. Semin. Cell Dev. Biol. 18, 716-731.
<https://doi.org/10.1016/j.semcdb.2007.09.003>
25. , K. (2000) Tripartite management of unfolded proteins in the endoplasmic reticulum. Cell 101, 451-454.
<https://doi.org/10.1016/S0092-8674(00)80855-7>
26. , S., Köse, S., Yersal, N. et al. (2022) Mesenchymal stem cells promote spermatogenesis in neonatal mice in vitro. Sci. Rep. 12, 11494.
<https://doi.org/10.1038/s41598-022-15358-5>
27. , C., Melton, D. W., Saunders, P. T. (2008) Do heat stress and deficits in DNA repair pathways have a negative impact on male fertility? Mol. Hum. Reprod. 14, 1-8.
<https://doi.org/10.1093/molehr/gam089>
28. , E. M., Costa, C. B., Barbosa Latorraca, L. et al. (2021) TUDCA modulates oxidative stress signaling in bovine oocytes. Front. Cell Dev. Biol. 9, 623852.
<https://doi.org/10.3389/fcell.2021.623852>
29. , M. Tavalaee, M. Drevet, J. R. et al. (2023) Role of endoplasmic reticulum stress in the male reproductive system. Cell J. 25, 437.
30. , S. V. S. (2020) Endoplasmic reticulum stress induced by toxic elements – a review of recent developments. Biol. Trace Elem. Res. 196, 10-19.
<https://doi.org/10.1007/s12011-019-01903-3>
31. , J., Santos, M. A. S., Fardilha, M. et al. (2020) Stress response pathways in the male germ cells and gametes. Mol. Hum. Reprod. 26, 1-13.
<https://doi.org/10.1093/molehr/gaz063>
32. , M. (2008) Endoplasmic reticulum stress responses. Cell. Mol. Life Sci. 65, 862-894.
<https://doi.org/10.1007/s00018-007-7383-5>
33. , B. P. (2018) The effects of heat on the testes of mammals. Anim. Reprod. 3, 81-91.
34. , A. M., Rizzoto, G., Kastelic, J. P. (2020) Amelioration of heat stress-induced damage to testes and sperm quality. Theriogenology 158, 84-96.
<https://doi.org/10.1016/j.theriogenology.2020.08.034>
35. Sharma, R., Agarwal, A. (2011) Spermatogenesis: an overview. In: Sperm Chromatin. Springer, eds. Zini, A., Agarwal, A., pp. 19-44, Springer, New York.
36. , C. Q., Sun, D. Z., Xu, Y. M. et al. (2019) ER calcium and paraquat-induced apoptosis in A549 cells. Mol. Med. Rep. 20, 2419-2425.
37. , I., Ron, D. (2011) Integrating mechanisms of apoptosis induced by ER stress. Nat. Cell Biol. 13, 184-190.
<https://doi.org/10.1038/ncb0311-184>
38. Tatar, M. (2024) Endoplasmic reticulum stress and infertility. In: Recent Researches in Health Sciences – 2024, eds. Deveci, H. A., Mor, N., pp. 95-106, Livre de Lyon, Lyon.
39. , P., Bujan, L., Multigner, L. et al. (1998) Occupational heat exposure and male fertility. Hum. Reprod. 13, 2122-2125.
<https://doi.org/10.1093/humrep/13.8.2122>
40. , J. K., Gani, A. R., Ramaiah, K. V. (2017) TUDCA mitigates ER stress-induced cell death. Sci. Rep. 7, 3831.
<https://doi.org/10.1038/s41598-017-03940-1>
41. , P., Ron, D. (2011) The unfolded protein response: from stress pathway to homeostasis. Science 334, 1081-1086.
<https://doi.org/10.1126/science.1209038>
42. , Q., Khaoustov, V. I., Chung, C. C. et al. (2002) Effect of TUDCA on ER stress-induced caspase-12 activation. Hepatology 36, 592-601.
<https://doi.org/10.1053/jhep.2002.35441>
43. , C. L., Liu, J. W., Wei, D. Z. et al. (2004) In vitro anti-tumor activity of chalcone derivatives. Pharmacol. Res. 50, 505-510.
<https://doi.org/10.1016/j.phrs.2004.05.004>
44. , J. et al. (2022) Endoplasmic reticulum stress promotes blood-testis barrier impairment in mice. Toxicology 473, 153193.
<https://doi.org/10.1016/j.tox.2022.153193>

