Folia Biologica
Journal of Cellular and Molecular Biology, Charles University 

Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune condition that causes patients pain and seriously affects their quality of life. Therefore, finding a diagnostic marker is of great significance for the prediction and early diagnosis of RA. This study included 105 patients with RA and 90 healthy volunteers. miR-383-5p was quantified by RT-qPCR. The diagnostic potential of miR-383-5p for RA was evaluated by the ROC curve. Chi-square analysis was performed to evaluate correlations between clinicopathological parameters and the miR-383-5p level in RA. Fibroblast-like synoviocytes (FLS) were isolated from synovial tissue. miR-383-5p was over-expressed or inhibited by transfection in FLS. ELISA was used to detect inflammatory factor expression. Pearson correlation analysis was utilized to detect the correlations. miR-383-5p was decreased in RA and may have a diagnostic value in RA. In patients with RA, miR-383-5p was closely related to erythrocyte sedimentation rate, C-reactive protein, anti-cyclic citrullinated peptide antibody positivity and rheumatoid factor. In addition, miR-383-5p was negatively correlated with inflammatory factors in RA. In FLS of RA, the over-expression of miR-383-5p decreased the inflammatory factors, while the inhibition of miR-383-5p increased the inflammatory factors. miR-383-5p may be associated with RA by involvement in the inflammatory response in FLS. Thus, miR-383-5p may show diagnostic potential in RA.

Supplementary materials: Supplementary Table (pdf)

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