Fol. Biol. 2000, 46, 11-15
Irradiation of Genetically Modified Plasmacytoma Vaccines Results in Upregulation of CD80 Molecule Expression, IL-2 Production and Higher Therapeutic Efficacy of the Vaccines
It has been found previously that irradiated, IL-2 gene-modified plasmacytoma (X63-m-IL-2) vaccines are more efficient in the therapy of the parental (X63-Ag8.653) plasmacytoma than live plasmacytoma vaccines. In this communication, we have demonstrated that irradiation of murine IL-2-producing plasmacytoma vaccines resulted in upregulation of CD80 molecule expression and IL-2 production. The expression of MHC class I antigens was not altered. The upregulation of the CD80 membrane molecule expression in X63-m-IL-2 cells was higher after irradiation with 150 Gy than after irradiation with 50 Gy. Comparable upregulation of the CD80 molecule expression has also been demonstrated after irradiation of the parental murine X63-Ag8.653 plasmacytoma cells. The results indicate that upregulation of the CD80 molecule expression and enhanced IL-2 production in irradiated X63-m-IL-2 cells was responsible for the higher therapeutic effectiveness of the irradiated plasmacytoma vaccine.
Funding
This work was supported by grants No. NC/5526-3 and NC/45011-3 from the Grant Agency of the Ministry of Health of the Czech Republic, No. 312/98/0826 and 312/99/0542 from the Grant Agency of the Czech Republic, and by Czech Terry Fox Foundation.
References
Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.