Fol. Biol. 2000, 46, 131-135
Genes Involved in the Destruction of Leukamic Cells by Induced Photosensitivity
Gene expression changes were observed in the HEL and HL-60 cell lines after the stimulation of protoporphyrin IX synthesis by ALA administration and photodynamic process induction. Isolated ribonucleic acids were radiolabelled by reverse transcription, and the cDNA obtained was hybridized to membrane macroarrays (Clontech 7742-1) containing 588 gene probes. Besides changes in the activity of genes supposed to be involved in the programmed cell death and DNA reparation processes, increased or diminished transcription activity was also observed in several other genes; the reason for this phenomenon was not clear. The activation of programmed-cell-death genes appeared after the ALA load application, indicating the toxic effect of ALA. The gene expression changes observed in the two cell lines differed substantially, only a few of them were common for both cell lines.
Keywords
HL-60, HEL, 5-aminolaevulinic acid, photodynamic process, gene expression, array technology.
Funding
This project was supported by the Internal Grant Agency of the Ministry of Health of the Czech Republic, grants Nos. NM 5901-3 and NC 5883-3.
References
Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.