Fol. Biol. 2001, 47, 106-107
Monoclonal Antibodies Specific to Polynuclear Aromatic Hydrocarbons
The interest in polycyclic aromatic hydrocarbons (PAHs), especially benzo[a]pyrene, in environmental samples (e.g. city aerosols) is derived from their known mutagenic potential (Topinka et al., 1998) and their adjuvant effect for the causation of allergies (Diaz-Sanchez, 1997; Bömmel et al., 2000). Analysis of PAHs is routinely performed by gas chromatography/mass spectrometry (GC/MS) or high performance liquid chromatography/fluorescence detection (HPLC/FD). However, these methods are time consuming, mainly due to complex procedures involved in the preparation of samples, which usually includes removal of disturbing matrix compounds and concentration of the target analytes. The objective of our project is to develop a rapid immunoassay which can be applied to screening of a large number of samples without the need for demanding sample preparation. In this communication we describe monoclonal antibodies BAP-13 and BAP-14 specific for a range of PAHS.
Keywords
PAH, polycyclic aromatic hydrocarbon, determination, ELISA, monoclonal antibody.
Funding
This work was supported by the grant No. ERB IC 15 СТ 98 0322 of the 4th Framework Program of the European Commission, INCO-COPERNICUS, and the grant OK381 (1999) from the Ministry of Education of the Czech Republic.
References
Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.