Fol. Biol. 2002, 48, 15-27
Protooncogene Bcl-2 Induces Apoptosis in Several Cell Lines
Using a recombinant vaccinia virus expressing protooncogene Bcl-2, we demonstrate opposite effects of the expressed Bcl-2 in two cell lines: apoptosis induction in BSC-40 cells and apoptosis prevention in HeLa G cells. The apparent molecular weight of the expressed Bcl-2, its amounts and its effects on the mitochondrial membrane potential are comparable in both cell lines, suggesting that the consequences of Bcl-2 expression depend on the cellular environment. To further support these findings we demonstrate the pro-apoptotic effect of the expressed Bcl-2 in several other cell lines.
Funding
The work was supported by the Grant Agency of the Czech Republic - project No. 310/96/0514, the Grant Agency of Charles University - project No. 232/95, the Grant Agency of the Ministry of Health of the Czech Republic - project No. NC5463-3, and the Ministry of Education of the Czech Republic - project No. VZ111100003.
References
Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.