Fol. Biol. 2002, 48, 43-50
DNA Vaccine against Friend Erythroleukaemia Virus
Plasmids carrying DNA copies of the gag and env genes of FV, which causes erythroleukaemia in susceptible mouse strains, were prepared. Expression of the cloned genes was confirmed by indirect immunofluoroscence in cells transfected in vitro. Immunization experiments were performed in DBA/2 mice. Animals were injected with three doses of plasmid either intramuscularly (100 μg DNA per dose) or intradermally (1 μg DNA per dose); in the latter case, a gene gun was used. The FV type A or P was used as a challenge. The immunization with gag- and env-derived vaccines resulted in protective immunity in a high proportion of mice.
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Funding
The research work was supported by grant No. 4069-3 of the Internal Grant Agency of the Ministry of Health of the Czech Republic, and by grant No. 312/99/0542 of the Grant Agency of the Czech Republic.
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