Fol. Biol. 2002, 48, 69-72
An ATP-Dependent Step Is Required for the Translocation of Microinjected Precursor mRNA into Nuclear Speckles
Nuclear speckles (speckles) represent a distinct nuclear compartment within the interchromatin space and are enriched in splicing factors. In a previous study (Melčák et al., 2001), it has been shown that the prespliceosomal assembly on microinjected splicing-competent precursor mRNA takes place in the speckles, and it has been suggested that the targeting of RNA into speckles consists of two interdependent steps, namely the diffusion process, followed by the energy-dependent translocation of RNA into the speckles. In the present study, we confirm the existence of these two steps and show that this latter translocation is ATP dependent.
Keywords
ATP-dependent step, HeLa cell nucleus, microinjected splicing-competent RNA, RNA translocation into nuclear speckles.
Funding
This work was supported by grants from the Grant Agency of the Czech Republic 304/00/1481 and 304/01/0729, from the Academy of Sciences of the Czech Republic IAA5039103 and AVOZ5039906, and from the Ministry of Education, Youth and Sports MSM: 111100003.
References
Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.