Folia Biologica
Journal of Cellular and Molecular Biology, Charles University 

Crossref logo

Fol. Biol. 2003, 49, 51-62

https://doi.org/10.14712/fb2003049020051

The 3' Untranslated Region of the Chicken c-src Protooncogene Modulates Gene Expression

K. Trejbalová1, J. Plachý1, P. Dezélée2, J. Geryk1, J. Svoboda1, G. Calothy2, Jiří Hejnar1

1Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
2Department of Retroviral and Molecular Oncogenetics, Institut Curie, Centre Universitaire, Orsay, France

Received February 2003
Accepted February 2003

Tight regulation of the Src tyrosine kinase activity is essential for a variety of cellular processes, namely transitions of the cell cycle. The peaks of Src activity are dependent on its posttranslational modifications as well as on the regulation of gene expression. The 3'UTRs of mRNAs are often crucial for rapid changes of the protein level. The chicken c-src 3'UTR effects on gene expression have been explored. The c-src 3'UTR decreased the in vivo tumorigenic potential of the src-activated mutants in chickens. This corresponds with the finding that the c-src 3'UTR reduced the Src protein and src mRNA levels and luciferase activity in vitro. Our results suggest that the chicken c-src 3'UTR plays a role in the negative control of gene expression, either transcriptionally or posttranscriptionally.

Funding

This work was supported by grants Nos. 204/02/0407 and 524/01/0866 from the Grant Agency of the Czech Republic.

References

41 live references