Folia Biologica
Journal of Cellular and Molecular Biology, Charles University 

Crossref logo

Fol. Biol. 2003, 49, 87-94

https://doi.org/10.14712/fb2003049020087

Morphological, Genetic and Functional Variability of a T-Cell Hybridoma Line

Balik M. Dzhambazov1, I. Teneva2, L. Koleva3, D. Asparuhova3, N. Popov3

1Section for Medical Inflammation Research, Department of Cell and Molecular Biology, Lund University, Lund, Sweden
2Junior Research Group, Molecular Animal Cell Toxicology, UFZ Centre for Environmental Research, Leipzig, Germany
3Cell Biology Laboratory, Department of Developmental Biology, University of Plovdiv, Plovdiv, Bulgaria

Received January 2003
Accepted February 2003

References

1. Altshuler, G. L., Dilwith, R., Sowek, J., Belfort, G. (1986) Hybridoma analysis at cellular level. Biotechnol. Bioeng. Symp. 17, 725-736.
2. Andersson, M., Holmdahl, R. (1990) Analysis of type II collagen-reactive T cells in the mouse. I. Different regulation of autoreactive vs. non-autoreactive anti-type II collagen T cells in the DBA/1 mouse. Eur. J. Immunol. 20, 1061-1066. <https://doi.org/10.1002/eji.1830200517>
3. Bäcklund, J., Treschow, A., Firan, M., Malmström, V., Issazadeh-Navikas, S., Ward, E. S., Holmdahl, R. (2002) Reversal of tolerance induced by transplantation of skin expressing the immunodominant T cell epitope of rat type II collagen entitles development of collagen-induced arthritis but not graft rejection. Eur. J. Immunol. 32, 1773-1783. <https://doi.org/10.1002/1521-4141(200206)32:6<1773::AID-IMMU1773>3.0.CO;2-Z>
4. Broddefalk, J., Bergquist, K. E., Kihlberg, J. (1996) Preparation of a glycopeptide analogue of type II collagen - use of acid labile protective groups for carbohydrate moieties in solid phase synthesis of O-linked glycopeptides. Tetrahedron Lett. 37, 3011-3014. <https://doi.org/10.1016/0040-4039(96)00456-X>
5. Castillo, F. J., Mullen, L. J., Grant, B. C., DeLeon, J., Thrift, J. C., Chang, L. W., Irving, J. M., Burke, D. J. (1994) Hybridoma stability. Dev. Biol. Stand. 83, 55-64.
6. Committee on Standardized Genetic Nomenclature for Mice (1972) Standard karyotype of the mouse, Mus musculus. J. Hered. 63, 69-72.
7. Corthay, A., Bäcklund, J., Broddefalk, J., Michaëlsson, E., Goldschmidt, T. J., Kihlberg, J., Holmdahl, R. (1998) Epitope glycosylation plays a critical role for T cell recognition of type II collagen in collagen-induced arthritis. Eur. J. Immunol. 28, 2580-2590. <https://doi.org/10.1002/(SICI)1521-4141(199808)28:08<2580::AID-IMMU2580>3.0.CO;2-X>
8. Demotz, S., Grey, H. M., Sette, A. (1990) The minimal number of class II MHC-antigen complexes needed for T cell activation. Science 249, 1028-1030. <https://doi.org/10.1126/science.2118680>
9. D'Eustachio, P., Pravtcheva, D., Marcu, K., Ruddle, F.H. (1980) Chromosomal location of the structural gene cluster encoding murine immunoglobulin heavy chains. J. Exp. Med. 151, 1545-1550. <https://doi.org/10.1084/jem.151.6.1545>
10. D'Eustachio, P., Bothwell, A. L., Takaro, T. K., Baltimore, D., Ruddle, F. H. (1981) Chromosomal location of structural genes encoding murine immunoglobulin lambda light chains. Genetics of murine lambda light chains. J. Exp. Med. 153, 793-800. <https://doi.org/10.1084/jem.153.4.793>
11. Fabris, V., Ernst, G., Lopes, E. C., Garcia, M., Hajos, S., Alvarez, E., Merani, S. (2001) Chromosome studies of murine T-cell lymphoid leukemia and derived cell lines. Cancer Genet. Cytogenet. 130, 62-67. <https://doi.org/10.1016/S0165-4608(01)00457-5>
12. Frame, K. K., Hu, W. (1989) The loss of antibody productivity in continuous culture of hybridoma cells. Biotechnol. Bioeng. 35, 469-476. <https://doi.org/10.1002/bit.260350504>
13. Gardner, J. S., Chiu, A. L., Maki, N. E., Harris, J. F. (1985) A quantitative stability analysis of human monoclonal antibody production by heteromyeloma hybridomas, using an immunofluorescent technique. J. Immunol. Methods 85, 335-346. <https://doi.org/10.1016/0022-1759(85)90142-5>
14. Harding, C. V., Unanue, E. R. (1990) Quantitation of antigenpresenting cell MHC class II/peptide complexes necessary for T-cell stimulation. Nature 346, 574-576. <https://doi.org/10.1038/346574a0>
15. Harrington, C. J., Paez, A., Hunkapiller, T., Mannikko, V., Brabb, T., Ahearn, M., Beeson, C., Goverman, J. (1998) Differential tolerance is induced in T cells recognizing distinct epitopes of myelin basic protein. Immunity 8, 571-580. <https://doi.org/10.1016/S1074-7613(00)80562-2>
16. Heath, C., Dilwith, R., Belfort, G. (1990) Methods for increasing monoclonal antibody production in suspension and entrapped cell cultures: biochemical and flow cytometric analysis as a function of medium serum content. J. Biotechnol. 15, 71-89. <https://doi.org/10.1016/0168-1656(90)90052-D>
17. Hurwitz, J. L., Herber-Katz, E., Hackett, C. J., Gerhard, W. (1984) Characterization of the murine TH response to influenza virus hemagglutinin: evidence for three major specificities. J. Immunol. 133, 3371-3377. <https://doi.org/10.4049/jimmunol.133.6.3371>
18. Köhler, G. (1980) Immunoglobulin chain loss in hybridoma lines. Proc. Natl. Acad. Sci. USA 77, 2197-2199. <https://doi.org/10.1073/pnas.77.4.2197>
19. Kontsek, P., Novák, M., Kontseková, E. (1988) Karyotype analysis of hybridomas producing monoclonal antibodies against different antigens. Folia Biol. (Praha) 34, 99-104.
20. Latek, R. R., Unanue, E. R. (1999) Mechanisms and consequences of peptide selection by the I-Ak class II molecule. Immunol. Rev. 172, 209-228. <https://doi.org/10.1111/j.1600-065X.1999.tb01367.x>
21. Malmström, V., Michaëlsson, E., Burkhardt, H., Mattsson, R., Vuorio, E., Holmdahl, R. (1996) Systemic versus cartilage-specific expression of a type II collagen-specific Tcell epitope determines the level of tolerance and susceptibility to arthritis. Proc. Natl. Acad. Sci. USA 93, 4480-4485. <https://doi.org/10.1073/pnas.93.9.4480>
22. Mamaeva, S. E. (1996) The patterns of the karyotypic evolution of cells in culture. Tsitologiia 38, 787-814.
23. Mansour, M. K., Schlesinger, L. S., Levitz, S. M. (2002) Optimal T cell responses to Cryptococcus neoformans mannoprotein are dependent on recognition of conjugated carbohydrates by mannose receptors. J. Immunol. 168, 2872-2879. <https://doi.org/10.4049/jimmunol.168.6.2872>
24. Meo, T., Johnson, J., Beechey, C. V., Andrews, S. J., Peters, J., Searle, A. G. (1980) Linkage analyses of murine immunoglobulin heavy chain and serum prealbumin genes establish their location on chromosome 12 proximal to the T (5;12) 31H breakpoint in band 12F1. Proc. Natl. Acad. Sci. USA 77, 550-553. <https://doi.org/10.1073/pnas.77.1.550>
25. Michaëlsson, E., Malmström, V., Reis, S., Engström, A., Burkhardt, H., Holmdahl, R. (1994) T cell recognition of carbohydrates on type II collagen. J. Exp. Med. 180, 745-749. <https://doi.org/10.1084/jem.180.2.745>
26. Miller, E. J. (1972) Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin. Biochemistry 11, 4903-4909. <https://doi.org/10.1021/bi00776a005>
27. Nagel, T., Kalden, J. R., Manger, B. (2000) Co-stimulation of IL-2 production by CD28 is independent of tyrosine-based signaling motifs in a murine T cell hybridoma. Eur. J. Immunol. 30, 1632-1637. <https://doi.org/10.1002/1521-4141(200006)30:6<1632::AID-IMMU1632>3.0.CO;2-Q>
28. Nakajima, A., Seroogy, C. M., Sandora, M. R., Tarner, I. H., Costa, G. L., Taylor-Edwards, C., Bachmann, M. H., Contag, C. H., Fathman, C. G. (2001) Antigen-specific T cell-mediated gene therapy in collagen-induced arthritis. J. Clin. Invest. 107, 1293-1301. <https://doi.org/10.1172/JCI12037>
29. Nowak, J. S. (1985) Loss of antibody production accompanied by chromosome loss in a cloned hybrid line secreting antibodies to sheep red blood cells. Experientia 41, 88-89. <https://doi.org/10.1007/BF02005890>
30. Ozturk, S. S., Palsson, B. O. (1990a) Physiological changes during the adaptation of hybridoma cells to low serum and serum-free media. Biotechnol. Bioeng. 37, 35-46. <https://doi.org/10.1002/bit.260370107>
31. Ozturk, S. S., Palsson, B. O. (1990b) Loss of antibody productivity during long-term cultivation of a hybridoma cell line in low serum and serum-free media. Hybridoma 9, 167-175. <https://doi.org/10.1089/hyb.1990.9.167>
32. Qadri, A., Ward, E. S. (2001) Activation of a T cell hybridoma by an alloligand results in differential effects on IL-2 secretion and activation-induced cell death. Eur. J. Immunol. 31, 3825-3832. <https://doi.org/10.1002/1521-4141(200112)31:12<3825::AID-IMMU3825>3.0.CO;2-O>
33. Rodova, M. A., Tsoi, L. A., Kushch, A. A., Novokhatskii, A. S. (1985) Karyological analysis of hybridoma lines producing monoclonal antibodies to viral antigens. Citol. Genet. 19, 425-428.
34. Rothfels, K. H., Siminovitch, L. (1958) An air-drying technique for flattening chromosomes in mammalian cells grown in vitro. Stain Technol. 33, 73-77. <https://doi.org/10.3109/10520295809111827>
35. Seabright, M. (1971) A rapid banding technique for human chromosomes. Lancet 7731, 971-972. <https://doi.org/10.1016/S0140-6736(71)90287-X>
36. Smith, B. D., Martin, G. R., Miller, E. J., Dorfman, A., Swarm, R. (1975) Nature of the collagen synthesized by a transplanted chondrosarcoma. Arch. Biochem. Biophys. 166, 181-186. <https://doi.org/10.1016/0003-9861(75)90378-1>
37. Surman, S., Lockey, T. D., Slobod, K. S., Jones, B., Riberdy, J. M., White, S. W., Doherty, P. C., Hurwitz, J. L. (2001) Localization of CD4+ T cell epitope hotspots to exposed strands of HIV envelope glycoprotein suggests structural influences on antigen processing. Proc. Natl. Acad. Sci. USA 98, 4587-4592. <https://doi.org/10.1073/pnas.071063898>
38. Swan, D., D'Eustachio, P., Leinwand, L., Seidman, J., Keithley, D., Ruddle, F. H. (1979) Chromosomal assignment of the mouse kappa light chain genes. Proc. Natl. Acad. Sci. USA 76, 2735-2739. <https://doi.org/10.1073/pnas.76.6.2735>
39. Tharakan, J. P., Chau, P. C. (1986) Serum free fed batch production of IgM. Biotechnol. Lett. 8, 457-462. <https://doi.org/10.1007/BF01025201>
40. Valitutti, S., Müller, S., Cella, M., Padovan, E., Lanzavecchia, A. (1995) Serial triggering of many T-cell receptors by a few peptide-MHC complexes. Nature 375, 148-151. <https://doi.org/10.1038/375148a0>
41. Volgareva, G. M. (1985) Karyologic research on murine B-cell hybridomas. Tsitologiia 27, 1394-1403.
42. Wellner, E., Gustafsson, T., Bäcklund J., Holmdahl, R., Kihlberg, J. (2000) Synthesis of a C-glycoside analogue of beta-D-galactosyl hydroxynorvaline and its use in immunological studies. Chembiochem. 1, 272-280. <https://doi.org/10.1002/1439-7633(20001117)1:4<272::AID-CBIC272>3.0.CO;2-W>
43. White, J., Blackman, M., Bill, J., Kappler, J., Marrack, P., Gold, D. P., Born, W. (1989) Two better cell lines for making hybridomas expressing specific T cell receptors. J. Immunol. 143, 1822-1825. <https://doi.org/10.4049/jimmunol.143.6.1822>
44. Wollweber, L., Münster, H., Hoffmann, S., Siller, K., Greulich, K. O. (2000) Early phase karyotype analysis of chromosome segregation after formation of mouse-mouse hybridomas with chromosome painting probes. Chromosome Res. 8, 37-44. <https://doi.org/10.1023/A:1009223102068>
45. Zhil'tsova, M. A., Trofimova, M. N., Novikov, V. V. (1989) Karyological analysis of hybridoma cells after prolonged cultivation. Zh. Mikrobiol. Epidemiol. Immunobiol. 6, 99-102. (Russian)
front cover

ISSN 0015-5500 (Print) ISSN 2533-7602 (Online)

Open access journal

Submissions

Archive