Folia Biologica
Journal of Cellular and Molecular Biology, Charles University 

Crossref logo

Fol. Biol. 2003, 49, 160-164

https://doi.org/10.14712/fb2003049040160

Cellular Localization of NGF and NGF Receptors in Aged Human Thymus

Tsvetana Marinova1, K. Velikova2, S. Philipov2, I. Stankulov3, G. Chaldakov3, L. Aloe4

1Department of Biology, Medical University, Sofia, Bulgaria
2Department of General and Clinical Pathology, Medical University, Sofia, Bulgaria
3Division of Cell Biology, Department of Forensic Medicine, Medical University, Varna, Bulgaria
4Institute of Neurobiology and Molecular Medicine, CNR, Rome, Italy

Received April 2003
Accepted May 2003

Recent evidence indicates that some thymic cells of developing and adult laboratory animals express the neurotrophin NGF and its low-affinity p75NTR and high-affinity TrkA receptor. Less is known as to whether the thymus of adult and aged humans express these markers. We hypothesize that the presence and distribution of immunopositive cells for NGF and NGF receptors undergo some alterations during the involution of human thymus. Specimens from normal thymuses of old individuals were obtained from autopsy and surgery cases, and examined immunocytochemically at the light and transmission electron microscopic level. The immunoreactivity of NGF, p75NTR, TrkA and cytokeratin was found in the epithelial thymocyte microenvironment. Our results provide the first ultrastructural evidence for NGF/receptor immunocytochemical localization in human thymus. They suggest a possible immunotrophic/immunoregulatory role of the NGF-p75NTR-TrkA system for T-cell development in human thymus during senile involution.

Funding

This work was supported by Grant No. 1/2002 from the Medical University, Sofia, Bulgaria, by the Institute of Neurobiology and Molecular Medicine, CNR, Rome, Italy and by Ricerca Finalizzata No. ICS 120.5/RF00.123.

References

20 live references