Fol. Biol. 2003, 49, 191-196
Gamma Irradiation Results in Phosphorylation of p53 at Serine-392 in Human T-Lymphocyte Leukaemia Cell Line MOLT-4
Exposure of human leukaemia MOLT-4 cells to ionizing irradiation led to apoptosis, which was detected by flow cytometric analysis and degradation of the nuclear lamina. The multiple signalling pathways triggered by either membrane or DNA damage play a critical role in radiation-induced apoptosis. The response to DNA damage is typically associated with the p53 protein accumulation. In this study, we proved that the transcriptionally active p53 variant occurs in the MOLT-4 cells and its abundance alteration is triggered in the γ-irradiated cell population concomitantly with phosphorylation at both the serine-392 and serine-15 residues. The p21 upregulation followed the p53 phosphorylation process in irradiated MOLT-4 cells.
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Funding
This work was supported by grants from the Czech Ministry of Defence (No. 03021100003) and from the Grant Agency of the Czech Republic (project No. 202/01/0016).
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This is an open-access article distributed under the terms of the Creative Commons Attribution License.