Fol. Biol. 2003, 49, 207-210
Effects of Adenosine on the Growth of Murine G:5:113 Fibrosarcoma Cells in Vitro
It has been observed that adenosine suppresses the growth of G:5:113 murine fibrosarcoma cells in vitro with EC50 of 178 mM. Changes in the cell cycle including decreased percentage of cells in S-phase, increased portion of cells in G0/G1-phase, as well as prolonged generation time were found to be responsible for the growth suppression. Dipyridamole, a drug inhibiting the cellular uptake of adenosine, enhanced the growth suppression induced with adenosine in concentrations of 100 and 200 µM. It follows from these results that the action of adenosine on the G:5:113 cells is extracellular, mediated by adenosine receptors. Elevation of extracellular adenosine might serve potentially as an anticancer therapeutic agent.
Keywords
G:5:113 fibrosarcoma cell line, cell growth, adenosine, dipyridamole.
Funding
This work was supported by the Grant Agency of the Czech Republic (grant No. 305/02/0423) and by the Grant Agency of the Academy of Sciences of the Czech Republic (grants Nos. S5004009 and K5011112).
References
Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.