Identification of HLA Alleles with Low or No Cell Surface Expression in the Czech Population

Bendukidze, Nina; Ivašková, E.; Zahlavová, L.; Slavcev, A.; Kupková, L.; Sajdlová, H.; Day, S.; Dunn, P. P. J.

doi:10.14712/fb2003049060227

Fol. Biol. 2003, 49, 227-229

https://doi.org/10.14712/fb2003049060227

Identification of HLA Alleles with Low or No Cell Surface Expression in the Czech Population

Nina Bendukidze^1,2, E. Ivašková³, L. Zahlavová⁴, A. Slavcev¹, L. Kupková³, H. Sajdlová¹, S. Day², P. P. J. Dunn²

¹Department of Immunogenetics, Institute for Clinical and Experimental Medicine (IKEM), Prague, Czech Republic
²H&I DNA Reference Laboratory, National Blood Service, Bristol, UK
³Czech Bone Marrow Donor Registry (CBMD), IKEM, Prague, Czech Republic
⁴Institute of Haematology and Blood Transfusion, Prague, Czech Republic

Received October 2003
Accepted October 2003

The presence of the A*24020102L allele is implicated in one donor from the CBMD who serologically was typed as A2; B44, B55; Cw1, Cw7. The DRB4*01030102N allele was identified in one healthy donor and in one patient with MDS during routine HLA class II DNA typing. The DRB4*01030102N allele was identified in the patient's father, who had CML, and was associated with the HLA-A3-B7-Cw7-DRB1*0701-DQB1*0303 haplotype, which is common for European populations. In order to avoid mistyping, both techniques, serology and molecular biology must be used for HLA typing, especially for cases where just one antigen appeared to be present using serological methods.