Fol. Biol. 2004, 50, 153-156
The Frequency of Alleles of the Pro12Ala Polymorphism in PPARγ2 Is Different between Healthy Controls and Patients with Type 2 Diabetes
The aim of this initial case-control study was to determine the association between common Pro12Ala polymorphism in the PPARγ2 gene and type 2 diabetes in the Czech Republic. Furthermore, the effect of this polymorphism on phenotypic characteristics and on levels of lipids (total cholesterol, HDL-cholesterol, LDL-cholesterol and triglycerides) was studied. One hundred thirty-three patients with type 2 diabetes and 97 control subjects were investigated. PCR and RFLP analysis were used for identification of individual genotypes. In the group of patients, three samples (2.26%) were identified as homozygous for the Ala/Ala genotype and 99 samples (74.44%) were homozygotes for the Pro/Pro genotype. Thirty-one samples (23.31%) were identified as Pro12Ala heterozygous. In the control group, six samples (6.19%) were homozygous for the Ala/Ala genotype and 61 samples (62.89%) were homozygotes for the Pro/Pro genotype. Thirty samples (30.93%) were identified as Pro12Ala heterozygous. The allele frequency for the Ala allele was lower in the type 2 diabetic group than in the control group (13.91% vs. 21.43%, P = 0.022). There was no difference (at P < 0.05) between the phenotypic characteristics (BMI, sex) studied in the group of patients according to the Pro12Ala genotype. There was no significant effect of the Pro12Ala polymorphism on lipid levels.
Keywords
PPARγ2, Pro12Ala polymorphism, type 2 diabetes, allele frequency, lipids.
Funding
Funded by the 3rd Faculty of Medicine, Charles University Prague; Grant Number: J13/98: 111200001.
References
Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.