Fol. Biol. 2004, 50, 167-173
Non-adherent Bone Marrow Cells Are a Rich Source of Cells Forming Bone in Vivo
Syngeneic, allogeneic and xenogeneic (rat) freshly isolated bone marow cells + stromal cell cultures maintained in vitro for 10-30 days, as well as nonadherent cells removed from these cultures on 3rd-4th day were injected into the kidney parenchyma of mice, immunosuppressed with hydrocortisone. In syngeneic grafts the immunosuppression was omitted. In all transplant systems bone tissue was formed inside the kidney with 20% to 32% variation. Bone produced by allogeneic and xenogeneic cells is subject to rejection when immunosuppression ceases, as the bone formed is of donor origin. The "floating" cells, regardless of the transplant system, normally discarded during media replacement, turned out to be efficient bone producers. This notion is of practical implication when bone marrow cells are used for bone healing.
Keywords
bone formation in kidneys, bone marrow, non-adherent bone marrow, stromal cell cultures, histocompatibility barrier.
Funding
This work was supported in part by grants from KBN (Commitee for Scientific Research) No. 4PO5C 047 14 and from Warsaw Medical Academy, No. 1M15/NK/2002.
References
Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.