Folia Biologica
Journal of Cellular and Molecular Biology, Charles University 

Crossref logo

Fol. Biol. 2005, 51, 82-84

https://doi.org/10.14712/fb2005051030082

The Effect of Gemcitabine in the Treatment of Rejection in Experimental Small Intestine Transplantation

Michal Kudla1, J. Kříž2, D. Janotová3, P. Girman2, H. Mergental1, M. Kočík1, E. Honsová3, M. Adamec1

1Department of Transplant Surgery, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
2Centre of Diabetes, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
3Department of Pathology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic

Received May 2005
Accepted May 2005

The aim of our study was to test the immunosuppressive effect of gemcitabine in monotherapy following heterotopic SBT in the rat. The BN and LEW rats were used as donors and recipients, respectively. Recipients were divided into 4 groups – group A without immunosuppression, group B treated with a therapeutic dose of tacrolimus, groups C and D treated with various doses of gemcitabine (100 and 150 µ µg/kg/day). Immunosuppression was administered once a day for 7 days after SBT, when the animals were sacrificed and a histological examination of grafts was performed. Only in group B no signs of acute rejection were seen. Significant differences (P < 0.01) were noted only between group B versus groups A, C, and D. No significant differences were demonstrated between groups A versus groups C, D and between group C versus group D. Monotherapy by gemcitabine (when administered at given doses) was not shown to be effective in preventing acute rejection in a rat model of heterotopic SBT.

Funding

This study was supported by the Institute for Clinical and Experimental Medicine (MZO 00023001).

References

11 live references