Fol. Biol. 2005, 51, 109-113
Immunogenicity and Protective Effect of a DNA Construct Encoding Certain Neutralizing Epitopes of Herpes Simplex Virus Type-1 Glycoprotein B
Much attention is presently focused on the vaccination with certain epitopes of an antigen. To further study the ability of neutralizing epitopes mapped in the first 1515 nucleotides of glycoprotein B of herpes simplex virus type-1 (gB-1) to induce neutralizing antibodies, a DNA immunization approach was employed. Vaccination of mice with a plasmid expressing the neutralizing epitopes induced humoral immune responses, although the antibody titre was significantly lower than that of antibodies induced by the full-length gB-1 gene. Furthermore, the plasmid DNA could not protect the mice against HSV-1 lethal challenge, but could significantly prolong the survival time compared to mock-vaccinated group.
Keywords
HSV-1, glycoprotein B gene, neutralizing epitopes, DNA immunization.
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