The Use of Comparative Duplex PCR in Monitoring of Patients with Non-Hodgkin's Lymphoma and Chronic Lymphocytic Leukaemia

Slavíčková, Alena; Forsterová, K.; Ivánek, R.; Černý, J.; Klener, P.

doi:10.14712/fb2005051060177

Fol. Biol. 2005, 51, 177-179

https://doi.org/10.14712/fb2005051060177

The Use of Comparative Duplex PCR in Monitoring of Patients with Non-Hodgkin's Lymphoma and Chronic Lymphocytic Leukaemia

Alena Slavíčková¹, K. Forsterová¹, R. Ivánek², J. Černý³, P. Klener¹

¹1st Clinic of Haemato-Oncology, Faculty of Medicine 1, Charles University, Prague, Czech Republic
²Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
³Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA

Received July 2005
Accepted October 2005

Various quantitative PCR approaches have been utilized during the last years to provide information about the treatment efficacy and the risk of recurrent disease in haematological malignancies. Apart from the frequently used real-time PCR, cost-saving modified standard PCR methods may be applied as well. This report evaluates the utility of the end-point comparative duplex PCR. We have used this method for monitoring of 35 patients with either NHL or CLL and observed a good correlation between quantitative molecular results and clinical outcome. There was also an agreement between comparative duplex PCR and real-time PCR in patients who were monitored by both methods. We therefore believe that use of this technique should be strongly considered instead of simple qualitative detection in monitoring of therapeutic outcome in NHL or CLL patients.