Folia Biologica
Journal of Cellular and Molecular Biology, Charles University 

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Fol. Biol. 2006, 52, 1-5

https://doi.org/10.14712/fb2006052010001

Effect of Lipopolysaccharide and Bleeding on the Expression of Intestinal Proteins Involved in Iron and Haem Transport

Jan Krijt1,2, M. Vokurka1,2, L. Šefc1,2, D. Ďuricová2, E. Nečas1,2

1Centre of Experimental Haematology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic
2Institute of Pathophysiology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

Received April 2006
Accepted May 2006

Haem carrier protein 1 (Hcp1) is a component of the haem-iron uptake pathway in the small intestine. Using quantitative real-time PCR, we examined the expression of Hcp1 and other intestinal irontransporting proteins in male C57BL/6 mice with experimentally altered iron homeostasis. Intestinal Hcp1 mRNA content was not significantly changed by iron overload (600 mg/kg); however, it was increased to 170 % of controls 72 h after withdrawal of 0.7 ml of blood; the same treatment increased intestinal Cybrd1 mRNA to 900 % of controls. LPS treatment (1 mg/kg, 6 h) decreased intestinal Hcp1 mRNA content to 66 % of controls and Flvcr mRNA content to 65 % of controls, while Cybrd1 mRNA, Dmt1 mRNA and Fpn1 mRNA decreased to 6 %, 43 % and 32 %, respectively. In 129SvJ mice with targeted disruption of the hemojuvelin (Hfe2) gene, which display very low expression of liver hepcidin, Cybrd1 mRNA content increased to 1040 %, Dmt1 mRNA content to 200 % and Fpn1 mRNA to 150 % when compared to wild-type mice; changes in Hcp1, Abcg2 and Flvcr mRNA content were only minor. Overall, these results suggest that, during inflammation, the intestinal haem-iron uptake pathway is not as strongly transcriptionally downregulated as the non-haem iron uptake pathway. A decrease in circulating hepcidin increases the expression of proteins participating in non-haem iron uptake, but has no significant effect on Hcp1 mRNA content.

Funding

This work was supported by grant MSM 0021620806 from the Ministry of Education, Youth and Sports of the Czech Republic.

References

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