Fol. Biol. 2007, 53, 33-36
Phenotypic Characterization of Porcine Interfollicular Keratinocytes Separated by Elutriation: a Technical Note
Separation of epidermal stem cells from other populations in suspensions of epidermal cells by sorting is hampered by a present lack of specific surface markers of this cell type. To address this issue we applied CCE combined with immunocytochemical phenotyping. On the basis of expression profiles for keratins (10, 14, and 19), nucleostemin, galectin-1 and epitopes reactive for this adhesion/growth-regulatory tissue lectin we identified a fraction of very small cells originating from the basal layer. The results demonstrated that CCE has potential merit for separation of epidermal cells to yield a population likely enriched in stem cells.
Keywords
elutriation, epidermal stem cell, galectin, keratin, keratinocyte, nucleostemin.
Funding
This study was supported by the Ministry of Education, Youth and Sports of the Czech Republic projects No. MSM0021620806 and No. 1M0021620803 and an EC Marie Curie Research Training Network grant (contract No. MRTN-CT-2005-019561).
References
Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.