Folia Biologica
Journal of Cellular and Molecular Biology, Charles University 

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Fol. Biol. 2007, 53, 33-36

https://doi.org/10.14712/fb2007053010033

Phenotypic Characterization of Porcine Interfollicular Keratinocytes Separated by Elutriation: a Technical Note

J. Klíma1,2, J. Motlík1,2, H.-J. Gabius3, Karel Smetana, Jr.1,4

1Center of Cell Tberapy and Tissue Repair, 2nd Faculty of Medicine, Charles University, Prague, Czech Republic
2Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic
3Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians University, Munich, Germany
4Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

Received December 2006
Accepted January 2007

References

1. André, S. Kaltner, H., Furuike, T., Nishimura, S.-I., Gabius, H.-J. (2004) Persubstituted cyclodextrin-based glycoclusters as inhibitors of protein-carbohydrate recognition using purified plant and mammalian lectins and wild-type and lectin-gene-transfected tumor cells as targets. Bioconjugate Chem. 15, 87-98. <https://doi.org/10.1021/bc0340666>
2. André, S., Kojima, S., Prahl, I., Lensch, M., Unverzagt, C., Gabius, H.-J. (2005) Introduction of extended LEC14-type branching into core-fucosylated biantennary N-glycan. FEBS J. 272, 1986-1998. <https://doi.org/10.1111/j.1742-4658.2005.04637.x>
3. Barrandon, J., Green, H. (1985) Cell size as a determinant of the clone-fomling ability of human keratinocytes. Proc. Natl. Acad. Sci. USA 82, 5390-5394. <https://doi.org/10.1073/pnas.82.16.5390>
4. Bauer, J. (1999) Advances in cell separation: recent developments in counterflow centrifugal elutriation and continuous flow cell separation. J. Chromatogr. B 722, 55-69. <https://doi.org/10.1016/S0378-4347(98)00308-9>
5. Dvořánková, B., Smetana, K. Jr., Chovanec, M., Lacina, L., Štork, J., Plzáková, Z., Galovičová, M., Gabius, H.-J. (2005) Transient expression of keratin K19 is induced in originally negative interfollicular epidermal cells by adhesion of suspended cells. Int. J. Mol. Med. 16, 525-531.
6. Gabius, H.-J. (2001) Glycohistochemistry the why and how of detection and localization of endogenous lectins. Anat. Histol. Embryol. 30, 3-31. <https://doi.org/10.1046/j.1439-0264.2001.00305.x>
7. Gabius, H.-J. (2006) Cell surface glycans: the why and how of their functionality as biochemical signals in lectin-mediated information transfer. Crit. Rev. Immunol. 26, 43-80. <https://doi.org/10.1615/CritRevImmunol.v26.i1.30>
8. Grabske, R. J., Lake, S., Gledhill, B. L., Meisstrich, M. L. (1975) Centrifugal elutriation: separation of spermatogenic cells on the basis of the sedimentation velocity. J. Cell. Physiol. 86, 177-189. <https://doi.org/10.1002/jcp.1040860119>
9. Kadri, T., Lataiilade, J. J., Doucet, C., Marie, A., Ernou, I., Bourin, P., Joubert-Caron, R., Caron, M., Lutomski, D. (2005) Proteomic study of Galectin-1 expression in human mesenchymal stem cells. Stem Cells Dev. 14, 204-212. <https://doi.org/10.1089/scd.2005.14.204>
10. Kucia, M., Reca, R., Campbell, F. R., Zuba-Surma, R., Majka, M., Ratajczak, J., Ratajczak, M. Z. (2006) A population of very small embryonic-like (VSEL) CXCR4+ SSEA-1+ OCT-4+ stem cells identified in adult bone marrow. Leukemia 20, 857-869. <https://doi.org/10.1038/sj.leu.2404171>
11. Lacina, L., Smetana, K. Jr., Dvořánková, B., Štork, J., Plzáková, Z., Gabius H.-J. (2006a) Immunocyto- and histochemical profiling of nucleostemin expression: marker of epidermal stem cells? J. Dermatol. Sci. 44, 73-80. <https://doi.org/10.1016/j.jdermsci.2006.08.008>
12. Lacina, L., Plzáková, Z., Smetana, K. Jr., Štork, J., Kaltner, H., André, S. (2006b) Glycophenotype of psoriatic skin. Folia Biol. (Praha) 52, 10-15.
13. Lahm, H., André, S., Hoeflich, A., Fischer, J. R., Sordat, B., Kaltner, H., Wolf, E., Gabius, H.-J. (2001) Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures. J. Cancer Res. Clin. Oncol. 127, 375-386. <https://doi.org/10.1007/s004320000207>
14. McEwen, C. R., Stalard, R. W., Juhos, E. T. (1968) Separation of biological particles by centrifugal elutriation. Anal. Biochem. 23, 369-377. <https://doi.org/10.1016/0003-2697(68)90228-5>
15. Michel M., Török, N., Bodnout, M.-J., Lussier, M., Gaudreau, P., Royal, A., Germain, L. (1996) Keratin 19 as a biochemical marker of skin stem cells in vivo and in vitro: keratin 19 expressing cells are differentially localized in function of anatomic sites, and their number varies with donor age and culture stage. J. Cell Sci. 109, 1017-1028. <https://doi.org/10.1242/jcs.109.5.1017>
16. Nagy, N., Legendre, H., Engels, O., André, S., Kaltner, H., Wasano, K., Zick, Y., Hector, J. C., Decaestecker, C., Gabius, H.-J., Salmon, I., Kiss, R. (2003) Refined prognostic evaluation in colon carcinoma using immunohistochemical galectin fingerprinting. Cancer 97, 1849-1858. <https://doi.org/10.1002/cncr.11268>
17. Pohl, R. J., Coomes, M. W., Sparks, R. W., Fouts, J. R. (1984) 7-ethoxycoumatin O-deethylation activity in viable basal and differentiated keratinocytes isolated from the skin of the hairless mouse. Drug. Metab. Dispos. 12, 25-34.
18. Purkrábková, T., Smetana, K. Jr., Dvořánková, B., Holíková, Z., Böck, C., Lensch, M., André, S., Pytlík, R., Liu, F.-T., Klíma J., Smetana, K., Motlík, J. , Gabius, H.-J. (2003) New aspects of galectin functionality in nuclei of cultured bone marrow stromal and epidermal cells: biotinylated galectins as tool to detect specific binding sites. Biol. Cell 95, 535-545. <https://doi.org/10.1016/j.biolcel.2003.08.002>
19. Rotblat, B., Niv, H., André, S., Kaltner, H., Gabius, H.-J., Kloog, Y. (2004) Galectin-1(L11A) predicted from a computed galectin-1 farnesyl-binding pocket selectively inhibits Ras-GTP. Cancer Res. 64, 3112-3118. <https://doi.org/10.1158/0008-5472.CAN-04-0026>
20. Saussez, S., Lorfevre, F., Nonclerq, D., Laurent, G., André, S., Journé, F., Kiss, R., Toubeau, G., Gabius, H.-J. (2006) Towards functional glycomics by localization of binding sites for tissue lectins: lectin histochemical reactivity for galectins during diethylstilbestrol-induced kidney tumorigenesis in male Syrian hamster. Histochem. Cell Biol. 126, 57-69. <https://doi.org/10.1007/s00418-006-0146-9>
21. Schwanke, U., Nabereit, A., Moog, R. (2006) Isolation of monocytes from whole blood-delived buffy coats by continuous counter-flow elutriation. J. Clin. Apher. 21, 153-157. <https://doi.org/10.1002/jca.20077>
22. Smetana, K. Jr., Dvořánková, B., Chovanec M., Bouček, J., Klíma, J., Motlík, J., Lensch, M., Kaltner, H., André, S., Gabius, H.-J. (2006) Nuclear presence of adhesion/growth-regulatory galectins in normal/malignant cells of squamous epithelial origin. Histochem. Cell Biol. 125, 171-182. <https://doi.org/10.1007/s00418-005-0074-0>
23. Vacanti, M. P., Roy, A., Cortiella, J., Bonasar, L., Vacanti, C. A. (2001) Identification and initial characterization of spore-like cells. J. Cell. Biochem. 80, 455-460. <https://doi.org/10.1002/1097-4644(20010301)80:3<455::AID-JCB180>3.0.CO;2-Z>
24. Villalobo, A., Nogales-González, A., Gabius, H.-J. (2006) A guide to signaling pathways connecting protein-glycan interaction with the emerging versatile effector functionality of mammalian lectins. Trends Glycosci. Glycotechnol. 18, 1-37. <https://doi.org/10.4052/tigg.18.1>
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