Fol. Biol. 2007, 53, 207-215
Downregulation of myc Promoter-Binding Protein 1 (MBP-1) in Growth-Arrested Malignant B cells
Normal human B lymphocytes are sensitive to the growth-inhibitory action of TGF-β1 whereas malignant B lymphoma cells are mostly resistant to TGF-β1 effects. We have shown in our previous work that, TGF-β1 treatment resulted in significant growth inhibition of the DoHH2 cell line. In the present study we showed that TGF-β1-induced growth arrest was associated with notable downregulation of the myc-binding protein-1 (MBP-1). Moreover, our results indicated that c-Myc overexpression in TGF-β1-arrested malignant B cells is mediated by binding of MBP-1, as a transcription repressor, to the (+118/+153) element of the promoter region of the myc gene.
Keywords
follicular lymphoma, transforming growth factor β1, myc-binding protein 1, two-dimensional gel electrophoresis, matrix-assisted laser desorption/ionisation time of flight mass spectrometry.
Funding
This work was supported by Grant of the Czech Science Foundation No. 301/05/P507 and Grants of the Ministry of Education, Youth and Sports of the Czech Republic MSM 0021620806 and LC 06044.
References
Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.