Fol. Biol. 2007, 53, 216-219
Multiplex Analysis of Cytokines Involved in Tumour Growth and Spontaneous Regression in a Rat Sarcoma Model
The aim of our study was to examine in vivo and in vitro cytokines produced by Lewis rat-derived R5-28 sarcoma cells. These cells produce rapidly growing tumours in approximately two weeks after subcutaneous inoculation. However, spontaneous tumour regression was noted in about 40% of animals. For an explanation of this phenomenon, we evaluated the profile of 19 cytokines during tumour growth and spontaneous regression by the use of "antibody array". To detect cytokines directly originated by the sarcoma, the R5-28 cells were cultivated in vitro and then both the supernatants and the cell lysates were analysed. Our experiments showed three cytokines (MCP-1, TIMP-1 and VEGF) to be produced by R5-28 cells in vitro. Moreover, in vivo, another three cytokines (TNF-α, β-NGF and LIX) were detected both in blood sera and tumour lysates, probably produced by immune and stromal cells during tumour growth. Changes in their expression after spontaneous regression are discussed.
Keywords
cytokines, sarcoma cells, spontaneous regression, antibody array.
Funding
This study was supported by the Czech Science Foundation (No. 524/04/0102, No. 310/03/H147 and No. 523/03/H076), the Grant Agency of the Academy of Sciences of the Czech Republic (IAA600450601, IAA500200510), Institutional Research Plan of the Institute of Animal Physiology and Genetics, v. v. i. (IRP IAPG No. AV0Z50450515), Grant Agency of Ministry of Education, Youth and Sports of the Czech Republic (MSM 6046070901), Institutional Research Concept AV0Z50200510 (IRC No. AV0Z50200510), and National Research Project NPVII 2B06130.
References
Copyright
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