Fol. Biol. 2007, 53, 220-221
The Effect of Interferon β-1a on MMP-2 and MMP-9 Proteolytic Activity
MMP-2 and MMP-9 play a significant role in the development of numerous diseases mainly with the inflammatory pathogenesis. One of the drugs exerting an effect on metalloproteinases is IFN-β. Previous studies showed that IFN β-1b can decrease MMP synthesis and moreover inhibit their proteolytic activity. The aim of our study was to analyse the influence of recombinant IFN β-1a (identical with natural IFN-β) on MMP-2 and MMP-9 activities. The gelatinolytic activity was evaluated with zymography in sera obtained from 10 healthy donors. After electrophoresis gels were incubated with or without IFN β-1a (2000 U/ml) for 18 h. We noticed a significant decrease of MMP-2/72 kDa (P = 0.0283) and the augmentation of MMP-9/92 kDa (P = 0.0042) activities after incubation with interferon. The elevation of MMP-9/92 kDa activity suggests that IFN β-1a can exhibit proinflammatory features besides well-known anti-inflammatory properties.
Keywords
IFN β-1a, MMP-2, MMP-9, zymography.
References
Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.