Fol. Biol. 2008, 54, 125-129

https://doi.org/10.14712/fb2008054040125

The Late-Stage Foetal Liver Microenvironment Is Essential for Later Sensitivity of B-Lymphopoiesis to Suppression by Oestrogens

Tereza Pelichovská1, K. T. Chang1,2, L. Šefc1, F. Savvulidi1, P. Broulík3, E. Nečas1

1Charles University in Prague, First Faculty of Medicine, Institute of Pathophysiology, Centre of Experimental Haematology, Prague, Czech Republic
2Academia Sinica, The Genomics Research Center, Stem Cell Program, Taipei, Taiwan
3Charles University in Prague, First Faculty of Medicine, Third Medical Department, Prague, Czech Republic

Received April 2008
Accepted August 2008

B-lymphopoiesis in FL differs notably from that of adult B-lymphopoiesis in being resistant to suppression by oestrogens due to the lack of expression of oestrogen receptors in B-cell progenitors and precursors. We have transplanted middle-stage FL cells (E14.5) to adult male mice and demonstrated that B-lymphopoiesis derived from FL cells remained resistant to suppression by exogenous oestrogen for several months compared to adult BM cells. This significant difference strongly suggests that the latestage FL environment exerts an inductive action on the haematopoietic stem cells and is mandatory for later sensitivity of B-lymphopoiesis to suppression by oestrogens. The results also provide the first in vivo functional confirmation of a differential responsiveness of FL- and adult BM-derived B-lymphopoiesis to suppression by oestrogens.

Funding

This work was supported by the research projects MSM 0021620806 and LC06044 granted by the Ministry of Education, Youth and Sports of the Czech Republic.

References

23 live references