Influence of Local Peripheral Temporary Ischaemia on Biochemical and Histological Effects in Small Intestine and Serum of Rats Following Abdominal Irradiation

Przybyszewski, Waldemar M.; Walichiewicz, P.; Widel, M.; Polaniak, R.; Snietura, M.; Maniakowski, Z.; Jacheć, W.

doi:10.14712/fb2008054060169

Folia Biologica > Archive > 2008, Vol. 54 > Issue 6 > Influence of Local Peripheral Temporary…

Fol. Biol. 2008, 54, 169-176

https://doi.org/10.14712/fb2008054060169

Influence of Local Peripheral Temporary Ischaemia on Biochemical and Histological Effects in Small Intestine and Serum of Rats Following Abdominal Irradiation

Waldemar M. Przybyszewski¹, P. Walichiewicz², M. Widel^1,3, R. Polaniak⁴, M. Snietura⁵, Z. Maniakowski⁶, W. Jacheć⁷

¹Department of Experimental and Clinical Radiobiology, Maria Sklodowska-Curie Memorial Cancer Centre and Institute of Oncology, Gliwice Branch, Gliwice, Poland
²Department of Radiotherapy, Maria Sklodowska-Curie Memorial Cancer Centre and Institute of Oncology, Gliwice Branch, Gliwice, Poland
³Institute of Automation, Silesian University of Technology, Gliwice, Poland
⁴Department of General Biochemistry, Zabrze, Silesian Medical University, Katowice, Poland
⁵Department of Pathology, Maria Sklodowska-Curie Memorial Cancer Centre and Institute of Oncology, Gliwice Branch, Gliwice, Poland
⁶Department of Medical Physics, Maria Sklodowska-Curie Memorial Cancer Centre and Institute of Oncology, Gliwice Branch, Gliwice, Poland
⁷Department of Cardiology, Zabrze, Silesian Medical University, Katowice, Poland

Received March 2008
Accepted December 2008

The local temporary ischaemia effect on radiation-induced lipid peroxidation, superoxide dismutase isoenzyme activities, and intestinal crypt number was estimated in male WAG-strain rats in vivo. The animals were irradiated in the abdomen area with doses of 2 Gy for ten consecutive days using a Philips ⁶⁰Co source. The calculated dose rate was 0.595 Gy/min. Local temporary ischaemia was induced by clamping the tail base before each irradiation. The parameters evaluated were: TBA-RS level and enzymatic activities of CuZnSOD, MnSOD in serum and jejunum. The number of jejunum crypts was assigned as a histopathologic parameter. The results showed a clear protection by ischaemic preconditioning for crypt survival. The difference in the number of crypts in irradiated animals with and without local temporary ischaemia was statistically significant (Student’s t-test P < 0.05). Also, significant enhancement of TBA-RS was observed in the serum of irradiated animals. Local temporary ischaemia application diminished the concentration of radiation-induced TBA-RS. The differences in the levels of TBA-RS in the serum were statistically significant (ANOVA P < 0.002). In contrast, there was no evident effect on the level of TBA-RS in tissue homogenates in any investigated groups. Some fluctuation of CuZnSOD isoenzyme activity in intestinal tissue was noted; however, the differences were not significant. Local temporary ischaemia had no influence on MnSOD activity in serum, and in both irradiated groups the behaviour of this isoenzyme was similar. Also, there were no differences in MnSOD activity measured in tissue homogenates. These findings support results of our previous in vivo studies, suggesting that local temporary ischaemia can prevent oxidative effects of fractionated radiotherapy.