Fol. Biol. 2008, 54, 180-186

https://doi.org/10.14712/fb2008054060180

Inhibition of CD44 Expression by Small Interfering RNA to Suppress the Growth and Metastasis of Ovarian Cancer Cells in Vitro and in Vivo

Chang-Zhong Li1, B. Liu2, Z.-Q. Wen1, C.-X. Wang3, H.-Y. Li1

1Department of Obstetrics and Gynecology, Shandong Provincial Hospital, Shandong University, Jinan, China
2Department of Medicine, Shandong Tumor Hospital, Jinan, China
3Department of Laboratory, Shandong Provincial Hospital, Jinan, China

Received July 2008
Accepted November 2008

References

1. Avin, E., Haimovich, J., Hollander, N. (2004) Anti-idiotype x anti-CD44 bispecific antibodies inhibit invasion of lymphoid organs by B cell lymphoma. J. Immunol. 173, 4736-4743. <https://doi.org/10.4049/jimmunol.173.7.4736>
2. Bourguignon, L. Y., Singleton, P. A., Diedrich, F., Stern, R., Gilad, E. (2004) CD44 interaction with Na+–H+exchanger (NHE1) creates acidic microenvironments leading to hyaluronidase-2 and cathepsin B activation and breast tumor cell invasion. J. Biol. Chem. 279, 26991-27007. <https://doi.org/10.1074/jbc.M311838200>
3. Bourguignon, L. Y., Gilad, E., Rothman, K., Peyrollier, K. (2005) Hyaluronan-CD44 interaction with IQGAP1 promo-tes Cdc42 and ERK signaling, leading to actin binding, Elk-1/estrogen receptor transcriptional activation, and ovarian cancer progression. J. Biol. Chem. 280, 11961-11972. <https://doi.org/10.1074/jbc.M411985200>
4. Chen, H. C. (2005) Boyden chamber assay. Methods Mol. Biol. 294, 15-22.
5. Draffin, J. E., McFarlane, S., Hill, A., Johnston, P. G., Waugh, D. J. (2004) CD44 potentiates the adherence of metastatic prostate and breast cancer cells to bone marrow endothelial cells. Cancer Res. 64, 5702-5711. <https://doi.org/10.1158/0008-5472.CAN-04-0389>
6. Ghatak, S., Misra, S., Toole, B. P. (2005) Hyaluronan con-stitutively regulates ErbB2 phosphorylation and signaling complex formation in carcinoma cells. J. Biol. Chem. 280, 8875-8883. <https://doi.org/10.1074/jbc.M410882200>
7. Greenlee, R. T., Murray, T., Bolden, S., Wingo, P. A. (2000) Cancer statistics, 2000. CA Cancer J. Clin. 50, 7-33. <https://doi.org/10.3322/canjclin.50.1.7>
8. Günthert, U., Hofmann, M., Rudy, W., Reber, S., Zöller, M., Haussmann, I., Matzku, S., Wenzel, A., Porta, H., Herrlich, P. (1991) A new variant of glycoprotein CD44 confers metastatic potential to rat carcinoma cells. Cell 65, 13-24. <https://doi.org/10.1016/0092-8674(91)90403-L>
9. Guo, Y., Ma, J., Wang, J., Che, X., Narula, J., Bigby, M., Wu, M., Sy, M. S. (1994) Inhibition of human melanoma growth and metastasis in vivo by anti-CD44 monoclonal antibody. Cancer Res. 54, 1561-1565.
10. Harada, N., Mizoi, T., Kinouchi, M., Hoshi, K., Ishii, S., Shiiba, K., Sasaki, I., Matsuno, S. (2001) Introduction of antisense CD44s cDNA down-regulates expression of overall CD44 isoforms and inhibits tumor growth and metastasis in highly metastatic colon carcinoma cells. Int. J. Cancer 91, 67-75. <https://doi.org/10.1002/1097-0215(20010101)91:1<67::AID-IJC1011>3.0.CO;2-D>
11. Hofmann, M., Rudy, W., Zöller, M., Tölg, C., Ponta, H., Herrlich, P., Günthert, U. (1991) CD44 splice variants confer metastatic behavior in rats: homologous sequences are expressed in human tumor cell lines. Cancer Res. 51, 5292-5297.
12. Jones, L. M., Gardner, M. J., Catterall, J. B., Turner, G. A. (1995) Hyaluronic acid secreted by mesothelial cells: a natural barrier to ovarian cancer cell adhesion. Clin. Exp. Metastasis 13, 373-380. <https://doi.org/10.1007/BF00121913>
13. Köbel, M., Gradhand, E., Zeng, K., Schmitt, W. D., Kriese, K., Lantzsch T., Wolters, M., Dittmer, J., Strauss, H. G., Thomssen, C. (2006) Ezrin promotes ovarian carcinoma cell invasion and its retained expression predicts poor prognosis in ovarian carcinoma. Int. J. Gynecol. Pathol. 25, 121-130. <https://doi.org/10.1097/01.pgp.0000185410.39050.ac>
14. Lakshman, M., Subramaniam, V., Wong, S., Jothy, S. (2005) CD44 promotes resistance to apoptosis in murine colonic epithelium. J. Cell Physiol. 203, 583-588. <https://doi.org/10.1002/jcp.20260>
15. Li, Y. H., Zhu, C. (1999) A modified Boyden chamber assay for tumor cell transendothelial migration in vitro. Clin. Exp. Metastasis 17, 423-429. <https://doi.org/10.1023/A:1006614232388>
16. Liao, H. X., Lee, D. M., Levesque, M. C., Haynes, B. F. (1995) N-terminal and central regions of the human CD44 extracellular domain participate in cell surface hyaluronan binding. J. Immunol. 155, 3938-3945. <https://doi.org/10.4049/jimmunol.155.8.3938>
17. McDonnel, A. C., Van Kirk, E. A., Isaak, D. D., Murdoch, W. J. (2005) Effects of progesterone on ovarian tumorigenesis in xenografted mice. Cancer Lett. 221, 49-53. <https://doi.org/10.1016/j.canlet.2004.09.011>
18. Omara-Opyene, A. L., Qiu, J., Shah, G. V., Iczkowski, K. A. (2004) Prostate cancer invasion is influenced more by expression of a CD44 isoform including variant 9 than by Muc18. Lab. Invest. 84, 894-907. <https://doi.org/10.1038/labinvest.3700112>
19. Ponta, H., Sherman, L., Herrlich, P. A. (2003) CD44: from adhesion molecules to signalling regulators. Nat. Rev. Mol. Cell Biol. 4, 33-45. <https://doi.org/10.1038/nrm1004>
20. Roscic-Mrkic, B., Fischer, M., Leemann, C., Manrique, A., Gordon, C. J., Moore, J. P., Proudfoot, A. E., Trkola, A.. (2003) RANTES (CCL5) uses the proteoglycan CD44 as an auxiliary receptor to mediate cellular activation signals and HIV-1 enhancement. Blood 102, 1169-1177. <https://doi.org/10.1182/blood-2003-02-0488>
21. Seiter, S., Arch, R., Reber, S., Komitowski, D., Hofmann, M., Ponta, H., Herrlich, P., Matzku, S., Zöller, M. (1993) Prevention of tumor metastasis formation by anti-variant CD44. J. Exp. Med. 177, 443-455. <https://doi.org/10.1084/jem.177.2.443>
22. Shi, Y., Tian, Y., Zhou, Y. Q., Ju, J. Y., Qu, L., Chen, S. L., Xian, S. L., Liu, Y., Zhu, L. P. (2007) Inhibition of malignant activities of nasopharyngeal carcinoma cells with high expression of CD44 by siRNA. Oncol. Rep. 18, 397-403.
23. Skubitz, A. P. (2002) Adhesion molecules. Cancer Treat. Res. 107, 305-329.
24. Strobel, T., Swanson, L., Cannistra, S. A. (1997) In vivo inhibition of CD44 limits intra-abdominal spread of a human ovarian cancer xenograft in nude mice: a novel role for CD44 in the process of peritoneal implantation. Cancer Res. 57, 1228-1232.
25. Stylianou, E., Jenner, L. A., Davies, M., Coles, G. A., Williams, J. D. (1990) Isolation, culture and characterization of human peritoneal mesothelial cells. Kidney Int. 37, 1563-1570. <https://doi.org/10.1038/ki.1990.150>
26. Subramaniam, V., Vincent, I. R., Gilakjan, M., Jothy, S. (2007) Suppression of human colon cancer tumors in nude mice by siRNA CD44 gene therapy. Exp. Mol. Pathol. 83, 332-340. <https://doi.org/10.1016/j.yexmp.2007.08.013>
27. Yang, W., Toffa, S. E., Lohn, J. W., Seifalian, A. M., Winslet, M. C. (2005) Malignant ascites increases the antioxidant ability of human ovarian (SKOV-3) and gastric adenocarcinoma (KATO-III) cells. Gynecol. Oncol. 96, 430-438. <https://doi.org/10.1016/j.ygyno.2004.10.016>
28. Yeo, T. K., Nagy J. A., Yeo, K. T., Dvorak, H. F., Toole, B. P. (1996) Increased hyaluronan at sites of attachment to mesentery by CD44-positive mouse ovarian and breast tumor cells. Am. J. Pathol. 148, 1733-1740.
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