Gamma-Radiation-Induced Phosphorylation of p53 on Serine 15 Is Dose-Dependent in MOLT-4 Leukaemia Cells

Tichý, Aleš; Záškodová, D.; Zoelzer, F.; Vávrová, J.; Šinkorová, Z.; Pejchal, J.; Österreicher, J.; Řezáčová, M.

doi:10.14712/fb2009055020041

Folia Biologica > Archive > 2009, Vol. 55 > Issue 2 > Gamma-Radiation-Induced Phosphorylation…

Fol. Biol. 2009, 55, 41-44

https://doi.org/10.14712/fb2009055020041

Gamma-Radiation-Induced Phosphorylation of p53 on Serine 15 Is Dose-Dependent in MOLT-4 Leukaemia Cells

Aleš Tichý^1,2, D. Záškodová¹, F. Zoelzer³, J. Vávrová², Z. Šinkorová², J. Pejchal⁴, J. Österreicher², M. Řezáčová¹

¹Charles University in Prague, Faculty of Medicine in Hradec Králové, Institute of Medical Biochemistry, Hradec Králové, Czech Republic
²University of Defence in Brno, Faculty of Health Sciences in Hradec Králové, Department of Radiobiology, Hradec Králové, Czech Republic
³University of South Bohemia in České Budějovice, Faculty of Health and Social Studies, Department of Radiology and Toxicology, České Budějovice, Czech Republic
⁴University of Defence in Brno, Faculty of Health Sciences in Hradec Králové, Centre of Advanced Studies, Hradec Králové, Czech Republic

Received April 2008
Accepted October 2008

Molecular indicators of the absorbed dose of ionizing radiation are powerful tools in biodosimetry. The studies reported here were undertaken with the motivation to find such a marker among the mo lecules involved in ataxia-telangiectasia mutated kinase-dependent signalling induced by ionizing radiation (ATM-kinase, checkpoint kinase-2, protein p53, and oncoprotein Mdm2). In our previous work on T-lymphocyte leukaemia MOLT-4 cells we described the mentioned molecules of ATM-dependent pathway and none of them showed a pronounced dosedependent response. Here we employed Western blotting and ELISA assay to investigate the response of post-translationally modified p53 (particularly phosphorylated on serine 15) after gamma-irradiation. We have found the amount of phosphorylated p53 to be homogenously increased after irradiation by the doses of 0.5 to 7.5 Gy. The dose-dependent response was pronounced especially after the doses up to 3.0 Gy. The presented data indicate that p53 phosphorylated on serine 15 might be used as a potential biodosimetric marker.