Cloning, Characterization, Chromosomal Mapping and Tissue Transcription Analysis of Porcine <i>CREB2</i> and <i>CREB3</i> Genes

Qi, M.; Lei, T.; Zhou, L.; Chen, X. D.; Long, H.; Long, Q. Q.; Zhang, R. R.; Yang, Z. Q.; Gan, Li

doi:10.14712/fb2009055040137

Folia Biologica > Archive > 2009, Vol. 55 > Issue 4 > Cloning, Characterization, Chromosomal…

Fol. Biol. 2009, 55, 137-144

https://doi.org/10.14712/fb2009055040137

Cloning, Characterization, Chromosomal Mapping and Tissue Transcription Analysis of Porcine CREB2 and CREB3 Genes

M. Qi^1,2, T. Lei², L. Zhou², X. D. Chen², H. Long², Q. Q. Long², R. R. Zhang², Z. Q. Yang², Li Gan¹

¹College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China
²Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China

Received September 2008
Accepted February 2009

CREB2 and CREB3 are two important members of the ATF/CREB family, which negatively and positively regulates CRE-dependent transcription in vitro. Here we report the cloning, chromosome mapping and tissue transcription analysis of CREB2 and CREB3 in pigs. The full-length coding sequence of CREB2 and CREB3 is 1047 bp and 1098 bp, encoding 348 and 365 amino acids, respectively. Porcine CREB3 comprises nine exons and eight introns, whereas CREB2 consists of three exons and two introns. CREB2 and CREB3 were cytogenetically assigned to porcine chromosome 5p and 1q28, respectively. Tissue transcription analysis revealed that both porcine CREB2 and CREB3 mRNA were ubi quitously detected in all examined tissues. Additionally, we cloned the 5’ flank genomic sequence of porcine CREB3 and characterized several putative transcription factor recognition sites including SP1, NF-κB, AP-1 and AP-2 in its promoter region. Our studies provide basic molecular information helpful for further investigation of the function of the two genes in pig models.

Keywords

cloning, CREB2, CREB3, porcine, tissue transcription.

Funding

This study was supported by the grants from Major Projects of National Science and Technology (2009 ZX 08009-151B), 973 (2006CB102100) Programme, 863 Programme (2008AA10 Z134, 2006AA10Z140), High Education Doctoral Subject Research Programme (20060504016), General Programme (30771585, 30600438) and Key Programme of National Natural Science Foundation of China.