Fol. Biol. 2009, 55, 153-158

https://doi.org/10.14712/fb2009055040153

Fibronectin-Replating Experiment: Procedure and Analysis

D. Rösel, F. Půta, Jan Brábek

Charles University in Prague, Faculty of Science, Department of Cell Biology, Prague, Czech Republic

Received February 2009
Accepted March 2009

References

1. Burridge, K., Turner, C. E., Romer, L. H. (1992) Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly. J. Cell Biol. 119, 893-903. <https://doi.org/10.1083/jcb.119.4.893>
2. Burridge, K., Chrzanowska-Wodnicka, M., Zhong, C. (1997) Focal adhesion assembly. Trends Cell Biol. 7, 342-347. <https://doi.org/10.1016/S0962-8924(97)01127-6>
3. Fournier, C., Brons, S., Taucher-Scholz, G. (2003) Quantification of chemiluminescent signals using photon-sensitive films or a CCD camera. Biotechniques 35, 284-290. <https://doi.org/10.2144/03352bi01>
4. Guan, J. L., Trevithick, J. E., Hynes, R. O. (1991) Fibronectin/integrin interaction induces tyrosine phosphorylation of a 120-kDa protein. Cell. Regul. 2, 951-964. <https://doi.org/10.1091/mbc.2.11.951>
5. Guan, J. L., Chen, H. C. (1996) Signal transduction in cell-matrix interactions. Int. Rev. Cytol. 168, 81-121. <https://doi.org/10.1016/S0074-7696(08)60883-9>
6. Hanks, S. K., Calalb, M. B., Harper, M. C., Patel, S. K. (1992) Focal adhesion protein-tyrosine kinase phosphorylated in response to cell attachment to fibronectin. Proc. Natl. Acad. Sci. USA 89, 8487-8491. <https://doi.org/10.1073/pnas.89.18.8487>
7. Hanks, S. K., Ryzhova, L., Shin, N. Y., Brábek, J. (2003) Focal adhesion kinase signaling activities and their implications in the control of cell survival and motility. Front. Biosc. 8, 982-996. <https://doi.org/10.2741/1114>
8. Kornberg, L., Earp, H. S., Parsons, J. T., Schaller, M., Juliano, R. L. (1992) Cell adhesion or integrin clustering increases phosphorylation of a focal adhesion-associated tyrosine kinase. J. Biol. Chem. 267, 23439-23442. <https://doi.org/10.1016/S0021-9258(18)35853-8>
9. Nagata, K., Izawa, I., Inagaki, M. (2001) A decade of siteand phosphorylation state-specific antibodies: recent advances in studies of spatiotemporal protein phosphorylation. Genes Cells 6, 653-664. <https://doi.org/10.1046/j.1365-2443.2001.00454.x>
10. Nojima, Y., Morino, N., Mimura, T., Hamasaki, K., Furuya, H., Sakai, R., Sato, T., Tachibana, K., Morimoto, C., Yazaki, Y., Hirai, H. (1995) Integrin-mediated cell adhesion promotes tyrosine phosphorylation of p130Cas, a Src homology 3-containing molecule having multiple Src homology 2-binding motifs. J. Biol. Chem. 270, 15398-153402. <https://doi.org/10.1074/jbc.270.25.15398>
11. Panetti, T. S. (2002) Tyrosine phosphorylation of paxillin, FAK, and p130CAS: effects on cell spreading and migration. Front. Biosci. 7, 143-150.
12. Poxton, I. R. (1990) Immunoblotting techniques. Curr. Opin. Immunol. 2, 905-909. <https://doi.org/10.1016/0952-7915(89)90176-3>
13. Schlaepfer, D. D., Hunter, T. (1998) Integrin signalling and tyrosine phosphorylation: just the FAKs? Trends Cell Biol. 8, 151-157. <https://doi.org/10.1016/S0962-8924(97)01172-0>
14. Sutcliffe, J. G., Shinnick, T. M., Green, N., Lerner, R. A. (1983) Antibodies that react with predetermined sites on proteins. Science 219, 660-666. <https://doi.org/10.1126/science.6186024>
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