Association of the <i>eNOS</i> 4a/b and -786T/C Polymormphisms with Coronary Artery Disease, Obesity and Diabetes Mellitus

Kincl, Vladimír; Vašků, A.; Meluzín, J.; Panovský, R.; Seménka, J.; Groch, L.

doi:10.14712/fb2009055050187

Folia Biologica > Archive > 2009, Vol. 55 > Issue 5 > Association of the eNOS 4a/b and…

Fol. Biol. 2009, 55, 187-191

https://doi.org/10.14712/fb2009055050187

Association of the eNOS 4a/b and -786T/C Polymormphisms with Coronary Artery Disease, Obesity and Diabetes Mellitus

Vladimír Kincl¹, A. Vašků², J. Meluzín¹, R. Panovský¹, J. Seménka¹, L. Groch¹

¹1st Department of Internal Medicine and Cardioangiology, St. Anne’s University Hospital, Faculty of Medicine, Masaryk University, Brno, Czech Republic
²Department of Pathophysiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic

Received January 2009
Accepted June 2009

The aim was to assess the relationship between eNOS 4a/b and -786T/C polymorphisms with coronary artery disease (CAD), obesity and diabetes mellitus. Total number of 1313 patients underwent coronary angiography, 939 had significant CAD (stenosis of ≥ 1 coronary artery ≥ 50%), 222 had smooth coronary arteries. Patients with insignificant atherosclerosis were excluded, the study finally comprised 1161 patients. The analysis of eNOS 4a/b and -786T/ C polymorphisms was performed by polymerase chain reaction. No significant interaction was found between -786T/C polymorphism and solitary CAD or CAD with diabetes and obesity. For 4a/b polymorphism, genotypes aa+ab were almost three times more frequent in diabetic patients without CAD versus patients without CAD and without diabetes – OR 2.79; P = 0.009, Pcorr = 0.03. In 4a/b polymorphism and CAD with obesity and diabetes: bb genotype was significantly more frequent: in patients with CAD, diabetes and obesity in comparison with obese diabetic patients without CAD (OR = 3.63, Pcorr = 0.05); in non-diabetic non-obese patients with CAD, versus diabetic and obese patients without CAD (OR = 3.38, Pcorr = 0.05); in obese non-diabetic patients without CAD vs. obese diabetic patients without CAD (OR = 5.91, Pcorr = 0.01); in patients without CAD, obesity and diabetes vs. obese diabetic patients without CAD (OR = 3.59, Pcorr = 0.05). The eNOS 4a/b polymorphism has significant association with diabetes mellitus in CAD-negative patients, and with CAD in combination with obesity and diabetes mellitus. No association between 4a/b or -786T/C polymorphism and solitary CAD was found.