Role of the <i>PPARα</i> Leu162Val and <i>PPARγ2</i> Pro12Ala Gene Polymorphisms in Weight Change after 2.5-Year Follow-up in Czech Obese Women

Aldhoon, Bashar; Zamrazilová, H.; Aldhoon Hainerová, I.; Šrámková, P.; Spálová, J.; Kunešová, M.; Bendlová, B.; Hainer, V.

doi:10.14712/fb2010056030116

Folia Biologica > Archive > 2010, Vol. 56 > Issue 3 > Role of the PPARα Leu162Val and…

Fol. Biol. 2010, 56, 116-123

https://doi.org/10.14712/fb2010056030116

Role of the PPARα Leu162Val and PPARγ2 Pro12Ala Gene Polymorphisms in Weight Change after 2.5-Year Follow-up in Czech Obese Women

Bashar Aldhoon^1,2, H. Zamrazilová², I. Aldhoon Hainerová³, P. Šrámková^2,4, J. Spálová², M. Kunešová², B. Bendlová², V. Hainer²

¹Department of Cardiology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
²Institute of Endocrinology, Prague, Czech Republic
³Charles University in Prague, Third Faculty of Medicine, Department of Pediatrics and Center for Research of Diabetes, Metabolism and Nutrition, Prague, Czech Republic
⁴Obesity Management Unit of the Clinical Centre ISCARE I.V.F., Prague, Czech Republic

Received August 2009
Accepted January 2010

The aim of this study was to investigate the possible effect of PPARα and PPARγ2 variants on weight and eating attitudes as well as on their changes after 2.5-year follow-up. The study was carried out in 246 Czech non-diabetic obese women (age 49.0 ± 11.9 years; BMI 38.1 ± 7.0 kg/m²). The comprehensive weight management programme included lowenergy diet, increased physical activity and lifestyle modification. Anthropometric parameters (body weight and height, waist and hip circumferences) and body composition were measured. The ThreeFactor Eating Questionnaire and Beck Depression Inventory were evaluated. At baseline and after the follow-up period, fasting levels of serum glucose, plasma adiponectin, ghrelin, leptin, and lipid profile were determined. The dependence of monitored parameters on the Pro12Ala in PPARγ2 and Leu162Val in PPARα and stage of the treatment (baseline; 2.5year follow-up) was evaluated using the repeated measures ANOVA model. The cohort was re-examined after 2.5 years, independent of regular checkups and adherence to lifestyle recommendation. Significant favourable changes in anthropometric indexes, lipid profile, leptin, ghrelin and adiponectin levels as well as in dietary restraint and hunger scores were revealed at 2.5-year check-up. However, no changes in the scores of disinhibition and depression were demonstrated. Despite several observed significant differences between carriers and non-carriers of the minor alleles at baseline and at the follow-up, the repeated measures ANOVA did not reveal any significant effect of the PPARα and PPARγ2 polymorphisms on anthropometric, biochemical, hormonal and psycho-behavioural characteristics, neither at baseline nor at the 2.5-year follow-up.

Keywords

PPARγ2, Pro12Ala polymorphism, obesity, PPARα, Leu162Val polymorphism, body mass index changes, psycho-behavioural factors and genetics.

Funding

The study was supported by grants from the research project of IGA MH CR NR/7800-4, IGA MH CR NS/10209-3, MSM No. 0021620814 and the Danone Institute 2008/2009.