Fol. Biol. 2010, 56, 124-130
Effect of Diplacone on LPS-Induced Inflammatory Gene Expression in Macrophages
Flavonoids are commonly studied for their anti-inflammatory effects; however, this is the first paper describing the possible antiphlogistic activity of a geranylated flavanone. This study focused on the ability of diplacone to modulate the gene expression of pro-inflammatory tumour necrosis factor α and monocyte chemoattractant protein 1, and of anti-inflammatory zinc finger protein 36. The action of diplacone was also compared with that of conventional drug indomethacin. Human monocyte-derived macrophages of the human monocytic leukaemia cell line were pretreated with diplacone or indomethacin. Subsequently, inflammatory reaction was induced by lipopolysaccharide, and changes of tumour necrosis factor α, monocyte chemoattractant protein 1 and zinc finger protein 36 gene expression at the transcriptional level were measured. In this model, diplacone significantly down-regulated the expression of tumour necrosis factor α and monocyte chemoattractant protein 1 and up-regulated the zinc finger protein 36 expression. This makes diplacone a promising molecule for treatment of the inflammatory stage of diseases. The effect of diplacone in decreasing lipopolysaccharide-induced inflammatory gene expression is in many ways similar to that of the conventional drug indomethacin.
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Funding
This project was supported by the Internal Grant Agency of the University of Veterinary and Pharmaceutical Sciences Brno, grant number 112/2008/FaF (to J.H.) and by the Ministry of Industry and Trade, grant number TANDEM FT-TA5/025 (to M.B.).
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Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.
