Fol. Biol. 2010, 56, 158-164

https://doi.org/10.14712/fb2010056040158

Advanced Rai Stage in Patients with Chronic Lymphocytic Leukaemia Correlates with Simultaneous Hypermethylation of Plural Tumour Suppressor Genes

Kristina Forsterová1, H. Votavová1, J. Schwarz2, J. Karban1, Č. Štuka1, M. Trněný1,2

1Charles University in Prague, First Faculty of Medicine, 1st Department of Medicine – Clinical Department of Haematology of the First Faculty of Medicine and General University Hospital, Prague, Czech Republic
2Institute of Haematology and Blood Transfusion, Prague, Czech Republic

Received January 2010
Accepted January 2010

Hypermethylation of CpG islands within gene promoters is one of various mechanisms of gene silencing involved in the pathogenesis of human cancer. By using methylation-specific polymerase chain reaction we explored aberrant promoter methylation of five tumour suppressor genes in 29 patients with chronic lymphocytic leukaemia. Aberrant methylation of DLC1, SHP1, p15 and p16 occurred, respectively, in 89.7 %, 70 %, 62.1 % and 31 % of patients at diagnosis. Lamin A/C was unmethylated in all the samples. Hypermethylation of at least one gene was detected in 96.6 % of patients. Concurrent methylation of two or more genes correlated with Rai stage at diagnosis.

Funding

This work was supported by a grant of the Ministry of Education, Youth and Sports of the Czech Republic (MSM 0021620808).

References

72 live references