Fol. Biol. 2010, 56, 211-217

https://doi.org/10.14712/fb2010056050211

The Expression of NADPH Oxidases and Production of Reactive Oxygen Species by Human Lung Adenocarcinoma Epithelial Cell Line A549

H. Kolářová1, L. Binó1,2, K. Pejchalová1, Lukáš Kubala1

1Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
2Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic

Received July 2009
Accepted August 2010

References

1. Baldus, S., Rudolph, V., Roiss, M., Ito, W. D., Rudolph, T. K., Eiserich, J. P., Sydow, K., Lau, D., Szocs, K., Klinke, A., Kubala, L., Berglund, L., Schrepfer, S., Deuse, T., Haddad, M., Risius, T., Klemm, H., Reichenspurner, H. C., Meinertz, T., Heitzer, T. (2006) Heparins increase endothelial nitric oxide bioavailability by liberating vessel-immobilized myeloperoxidase. Circulation 113, 1871-1878. <https://doi.org/10.1161/CIRCULATIONAHA.105.590083>
2. Bartosz, G. (2006) Use of spectroscopic probes for detection of reactive oxygen species. Clin. Chim. Acta 368, 53-76. <https://doi.org/10.1016/j.cca.2005.12.039>
3. Chapman, K. E., Sinclair, S. E., Zhuang, D., Hassid, A., Desai, L. P., Waters, C. M. (2005) Cyclic mechanical strain increases reactive oxygen species production in pulmonary epithelial cells. Am. J. Physiol. Lung Cell Mol. Physiol. 289, L834-841. <https://doi.org/10.1152/ajplung.00069.2005>
4. Daiber, A., August, M., Baldus, S., Wendt, M., Oelze, M., Sydow, K., Kleschyov, A. L., Munzel, T. (2004) Measurement of NAD(P)H oxidase-derived superoxide with the luminol analogue L-012. Free Radic. Biol. Med. 36, 101-111. <https://doi.org/10.1016/j.freeradbiomed.2003.10.012>
5. Dvorakova, J., Velebny, V., Kubala, L. (2008) Hyaluronan influence on the onset of chondrogenic differentiation of mesenchymal stem cells. Neuro Endocrinol. Lett. 29, 685-690.
6. Fink, K., Duval, A., Martel, A., Soucy-Faulkner, A., Grandvaux, N. (2008) Dual role of NOX2 in respiratory syncytial virusand sendai virus-induced activation of NF-κB in airway epithelial cells. J. Immunol. 180, 6911-6922. <https://doi.org/10.4049/jimmunol.180.10.6911>
7. Fischer, H., Gonzales, L. K., Kolla, V., Schwarzer, C., Miot, F., Illek, B., Ballard, P. L. (2007) Developmental regulation of DUOX1 expression and function in human fetal lung epithelial cells. Am. J. Physiol. Lung Cell Mol. Physiol. 292, L1506-1514. <https://doi.org/10.1152/ajplung.00029.2007>
8. Freitas, M., Lima, J. L., Fernandes, E. (2009) Optical probes for detection and quantification of neutrophils’ oxidative burst. A review. Anal. Chim. Acta 649, 8-23. <https://doi.org/10.1016/j.aca.2009.06.063>
9. Gallova, L., Kubala, L., Ciz, M., Lojek, A. (2004) IL-10 does not affect oxidative burst and expression of selected surface antigen on human blood phagocytes in vitro. Physiol. Res. 53, 199-208. <https://doi.org/10.33549/physiolres.930440>
10. Geiszt, M., Witta, J., Baffi, J., Lekstrom, K., Leto, T. L. (2003) Dual oxidases represent novel hydrogen peroxide sources supporting mucosal surface host defense. FASEB J. 17, 1502-1504. <https://doi.org/10.1096/fj.02-1104fje>
11. Goyal, P., Weissmann, N., Rose, F., Grimminger, F., Schafers, H. J., Seeger, W., Hanze, J. (2005) Identification of novel Nox4 splice variants with impact on ROS levels in A549 cells. Biochem. Biophys. Res. Commun. 329, 32-39. <https://doi.org/10.1016/j.bbrc.2005.01.089>
12. Jia, L., Xu, M., Zhen, W., Shen, X., Zhu, Y., Wang, W., Wang, X. (2008) Novel anti-oxidative role of calreticulin in protecting A549 human type II alveolar epithelial cells against hypoxic injury. Am. J. Physiol. Cell Physiol. 294, C47-55. <https://doi.org/10.1152/ajpcell.00019.2007>
13. Krejcova, D., Prochazkova, J., Kubala, L., Pachernik, J. (2009) Modulation of cell proliferation and differentiation of human lung carcinoma cells by the interferon-α. Gen. Physiol. Biophys. 28, 294-301. <https://doi.org/10.4149/gpb_2009_03_294>
14. Kubala, L., Ciz, M., Soska, V., Cerny, J., Lojek, A. (2002) Influence of polysulfone and hemophan hemodialysis membranes on phagocytes. Gen. Physiol. Biophys. 21, 367-380.
15. Luxen, S., Belinsky, S. A., Knaus, U. G. (2008) Silencing of DUOX NADPH oxidases by promoter hypermethylation in lung cancer. Cancer Res. 68, 1037-1045. <https://doi.org/10.1158/0008-5472.CAN-07-5782>
16. Malec, V., Gottschald, O. R., Li, S., Rose, F., Seeger, W., Hanze, J. (2010) HIF-1 α signaling is augmented during intermittent hypoxia by induction of the Nrf2 pathway in NOX1-expressing adenocarcinoma A549 cells. Free Radic. Biol. Med. 48, 1626-1635. <https://doi.org/10.1016/j.freeradbiomed.2010.03.008>
17. Nishinaka, Y., Aramaki, Y., Yoshida, H., Masuya, H., Sugawara, T., Ichimori, Y. (1993) A new sensitive chemiluminescence probe, L-012, for measuring the production of superoxide anion by cells. Biochem. Biophys. Res. Commun. 193, 554-559. <https://doi.org/10.1006/bbrc.1993.1659>
18. Pacquelet, S., Lehmann, M., Luxen, S., Regazzoni, K., Frausto, M., Noack, D., Knaus, U. G. (2008) Inhibitory action of NoxA1 on dual oxidase activity in airway cells. J. Biol. Chem. 283, 24649-24658. <https://doi.org/10.1074/jbc.M709108200>
19. Pavelkova, M., Kubala, L. (2004) Luminol-, isoluminoland lucigenin-enhanced chemiluminescence of rat blood phagocytes stimulated with different activators. Luminescence 19, 37-42. <https://doi.org/10.1002/bio.754>
20. Serrander, L., Cartier, L., Bedard, K., Banfi, B., Lardy, B., Plastre, O., Sienkiewicz, A., Forro, L., Schlegel, W., Krause, K. H. (2007) NOX4 activity is determined by mRNA levels and reveals a unique pattern of ROS generation. Biochem. J. 406, 105-114. <https://doi.org/10.1042/BJ20061903>
21. Tarpey, M. M., Fridovich, I. (2001) Methods of detection of vascular reactive species: nitric oxide, superoxide, hydrogen peroxide, and peroxynitrite. Circ. Res. 89, 224-236. <https://doi.org/10.1161/hh1501.094365>
22. van der Vliet, A. (2008) NADPH oxidases in lung biology and pathology: host defense enzymes, and more. Free Radic. Biol. Med. 44, 938-955. <https://doi.org/10.1016/j.freeradbiomed.2007.11.016>
23. Zhang, Z., Huang, C., Li, J., Leonard, S. S., Lanciotti, R., Butterworth, L., Shi, X. (2001) Vanadate-induced cell growth regulation and the role of reactive oxygen species. Arch. Biochem. Biophys. 392, 311-320. <https://doi.org/10.1006/abbi.2001.2464>
24. Zhou, M., Diwu, Z., Panchuk-Voloshina, N., Haugland, R. P. (1997) A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. Anal. Biochem. 253, 162-168. <https://doi.org/10.1006/abio.1997.2391>
front cover

ISSN 0015-5500 (Print) ISSN 2533-7602 (Online)

Open access journal

Submissions

Archive