Fol. Biol. 2010, 56, 195-200

https://doi.org/10.14712/fb2010056050195

Reversal of Hypermethylation and Reactivation of the RARβ2 Gene by Natural Compounds in Cervical Cancer Cell Lines

A. K. Jha1, M. Nikbakht1, G. Parashar1, A. Shrivastava2, N. Capalash1, Jagdeep Kaur1

1Department of Biotechnology, Panjab University, Chandigarh, India
2Department of Zoology, University of Delhi, Delhi, India

Received July 2010
Accepted September 2010

References

1. Angeles, E., Vázquez-Valadez, V. H., Vázquez-Valadez, O., Velázquez-Sánchez, A. M., Ramírez, A., Martínez, L., Díaz-Barriga, S., Romero-Rojas, A., Cabrera, G., LópezCastañares, R., Dueñas-González, A. (2005) Computational studies of 1-hydrazinophtalazine (hydralazine) as antineoplastic agent. Docking studies on methyltransferase. Lett. Drug Des. Discov. 4, 282-286. <https://doi.org/10.2174/1570180054038413>
2. Dorai, T., Aggarwal, B. (2004) Role of chemopreventive agents in cancer therapy. Cancer Lett. 215, 129-40. <https://doi.org/10.1016/j.canlet.2004.07.013>
3. Fang, M. Z., Wang, Y., Ai, N., Hou, Z., Sun, Y., Lu, Y., Welsh, W., Yang, C. S. (2003) Tea polyphenol (-)-epigallocatechin3-gallate inhibits DNA methyltransferase and reactivates methylation-silenced genes in cancer cell lines. Cancer Res. 63, 7563-7570.
4. Fang, M., Chen, D., Yang, C. S. (2007) Dietary polyphenols may affect DNA methylation. J. Nutr. 137, 223S-228S. <https://doi.org/10.1093/jn/137.1.223S>
5. Freshney, R. I. (1994) Culture of Animal Cells. A Manual of Basic Techniques, Third Edition. John Wiley & Sons, Inc. New York, NY.
6. Gong, J., Traganos, F., Darzynkiewicz, Z. (1994) A selective procedure for DNA extraction from apoptotic cells applicable for gel electrophoresis and flow cytometry. Anal. Biochem. 218, 314-319. <https://doi.org/10.1006/abio.1994.1184>
7. Gonzalez, A. D., Lizano, M., Candelaria, M., Cetina, M. L., Arce, C., Cervera, E. (2005) Epigenetics of cervical cancer. An overview and therapeutic perspectives. Mol. Cancer 4, 38. <https://doi.org/10.1186/1476-4598-4-38>
8. Heckenkamp, J., Leszczynski, D., Schiereck, J., Kung, J., La Muraglia, G. M. (1999) Different effects of photodynamic therapy and gamma irradiation on vascular smooth muscle cells and matrix: implications for inhibiting restenosis. Arterioscler. Thomb. Vasc. Biol. 19, 2154-2161. <https://doi.org/10.1161/01.ATV.19.9.2154>
9. Herman, J. G., Graff, J. R., Myohanen, S., Nelkin, B. D., Baylin, S. B. (1996) Methylation-specific PCR: A novel PCR assay for methylation status of CpG islands, Proc. Natl. Acad. Sci. USA 93, 9821-9826. <https://doi.org/10.1073/pnas.93.18.9821>
10. Hoque, M. O., Begum, S., Topaloglu, O., Jeronimo, C., Mambo, E., Westra, W. H., Califano, J. A., Sidransky, D. (2004) Quantitative detection of promoter hypermethylation of multiple genes in the tumor, urine, and serum DNA of patients with renal cancer. Cancer Res. 64, 5511-5517. <https://doi.org/10.1158/0008-5472.CAN-04-0799>
11. King-Batoon, A., Lesczynska, J. M., Klein, C. B. (2008) Modulation of gene methylation by genistein or lycopene in breast cancer cells. Environ. Mol. Mutagen. 49, 36-45. <https://doi.org/10.1002/em.20363>
12. Lee, W. J., Shim, J. Y., Zhu, B. T. (2005) Mechanisms for the inhibition of DNA methyltransferases by tea catechins and bioflavonoids. Mol. Pharmacol. 68, 1018-1030. <https://doi.org/10.1124/mol.104.008367>
13. Paluszczak, J., Krajka-Kuzniak, V., Baer-Dubowska, W. B. (2010) The effect of dietary polyphenols on the epigenetic regulation of gene expression in MCF7 breast cancer cells. Toxicol. Lett. 192, 119-125. <https://doi.org/10.1016/j.toxlet.2009.10.010>
14. Prusty, B., Das, B. (2005) Constitutive activation of transcription factor AP-1 in cervical cancer and suppression of HPV transcription and AP-1 activity in HeLa cells by curcumin. Int. J. Cancer 113, 951-960. <https://doi.org/10.1002/ijc.20668>
15. Zhongfa, L., Zhiliang, X., William, J., Ryan, E. P., Shujun, L., Jianhua, Y., Pui-kai, L., Jiayuh, L., Jame, R. F., Marcucci, G. M., Chenglong, L., Chan, K. K. (2009) Curcumin is a potent DNA hypomethylation agent. Bioorg. Med. Chem. Lett. 19, 706-709.
front cover

ISSN 0015-5500 (Print) ISSN 2533-7602 (Online)

Open access journal

Submissions

Archive