Fol. Biol. 2011, 57, 125-132
Comparative Analysis of the Nuclear Presence of Adhesion/Growth-Regulatory Galectins and Reactivity in the Nuclei of Interphasic and Mitotic Cells
Nuclear galectins participate in splicing of pre-mRNA. In this study we detected galectins-1, -2, -3 and -7 and their glycoligands in three types of cells: fibroblasts, cancer epithelial cells and melanoma cells. The results demonstrated that the nuclear expression of distinct types of galectins and their ligands in interphasic nuclei is dependent on the cell type. The extensive binding of labelled galectins-1 and -2 to mitotic cells (around chromosomes, in mitotic spindle and in bridge connecting both daughter cells) suggests their role during the cell division.
Keywords
lectin, mitose, cell nucleus, Ki67.
Funding
This study was supported by the Ministry of Education, Youth and Sport of the Czech Republic, projects Nos. MSM 0021620806 and 1M0538, and by the Charles University project for support of specific university research.
References
Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.
