Folia Biologica
Journal of Cellular and Molecular Biology, Charles University 

Crossref logo

Fol. Biol. 2011, 57, 125-132

https://doi.org/10.14712/fb2011057030125

Comparative Analysis of the Nuclear Presence of Adhesion/Growth-Regulatory Galectins and Reactivity in the Nuclei of Interphasic and Mitotic Cells

O. Kodet1,2, B. Dvořánková2,3, L. Lacina1,2,3, S. André4, H. Kaltner4, H.-J. Gabius4, Karel Smetana, Jr.2,3

1Department of Dermatovenereology and Venerology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic
2Institute of Anatomy, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
3Centre of Cell Therapy and Tissue Repair, Second Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
4Ludwig-Maximilians-University Munich, Faculty of Veterinary Medicine, Institute of Physiological Chemistry, Munich, Germany

Received December 2010
Accepted December 2010

References

1. André, S., Pei, Z., Siebert, H.-C., Ramström, O., Gabius, H.J. (2006) Glycosyldisulfides from dynamic combinatorial libraries as O-glycoside mimetics for plant and mammalian lectins: their reactivities in solid-phase and cell assays and conformational analysis by molecular dynamics simulations. Bioorg. Med. Chem. 14, 6314-6323. <https://doi.org/10.1016/j.bmc.2006.05.045>
2. André, S., Sanchez-Ruderisch, H., Nakagawa, H., Buchholz, M., Kopitz, J., Forberich, P., Kemmner, W., Böck, C., Deguchi, K., Detjen, K. M., Wiedenmann, B., von Knebel Doeberitz, M., Gress, T. M., Nishimura, S.-I., Rosewicz, S., Gabius, H.-J. (2007) Tumor suppressor p16INK4a: modulator of glycomic profile and galectin-1 expression to increase susceptibility to carbohydrate-dependent induction of anoikis in pancreatic carcinoma cells. FEBS J. 274, 3233-3256. <https://doi.org/10.1111/j.1742-4658.2007.05851.x>
3. André, S., Sansone, F., Kaltner, H., Casnati, A., Kopitz, J., Gabius, H.-J., Ungaro, R. (2008) Calix[n]arene-based glycoclusters: bioactivity of thiourea-linked galactose/lactose moieties as inhibitors of binding of medically relevant lectins to a glycoprotein and cell-surface glycoconjugates and selectivity among human adhesion/growth-regulatory galectins. Chembiochem 9, 1649-1461. <https://doi.org/10.1002/cbic.200800035>
4. Austin, C. M, Bellini, M. (2010) The dynamic landscape of the cell nucleus. Mol. Rep. Dev. 77, 19-28. <https://doi.org/10.1002/mrd.21088>
5. Bártová, E., Horáková, A. H., Uhlířová, R., Raška, I., Galiová, G., Orlová, D., Kozubek, S. (2010) Structure and epigenetics of nucleoli in comparison with non-nucleolar compartments. J. Histochem. Cytochem. 58, 391-403. <https://doi.org/10.1369/jhc.2009.955435>
6. Čada, Z., Chovanec, M., Smetana Jr., K., Betka, J., Lacina, L., Plzák, J., Kodet, R., Štork, J., Lensch, M., Kaltner, H., André, S., Gabius, H.-J. (2009a) Galectin-7: will the lectin’s activity establish clinical correlations in head and neck squamous cell and basal cell carcinomas? Histol. Histopathol. 24, 41-48.
7. Čada, Z., Smetana Jr., K., Lacina, L., Plzáková, Z., Štork, J., Kaltner, H., Russwurm, R., Lensch, M., André, S., Gabius, H.-J. (2009b) Immunohistochemical fingerprinting of the network of seven adhesion/growth-regulatory lectins in human skin and detection of distinct tumour-associated alterations. Folia Biol. (Praha) 55, 145-52.
8. Castano, E., Philimonenko, V. V., Kahle, M., Fukalová, J., Kalendová, A., Yildirim, S., Dzijak, R., Dingová-Krásná, H., Hozák, P. (2010) Actin complexes in the cell nucleus: new stones in an old field. Histochem. Cell Biol. 133, 607-626. <https://doi.org/10.1007/s00418-010-0701-2>
9. Chovanec, M., Smetana Jr., K., Dvořánková, B., Plzáková, Z., André, S., Gabius, H.-J. (2004) Decrease of nuclear reactivity to growth-regulatory galectin-1 in senescent human keratinocytes and detection of non-uniform staining profile alterations upon prolonged culture for galectin-1 and -3. Anat. Histol. Embryol. 33, 348-354. <https://doi.org/10.1111/j.1439-0264.2004.00568.x>
10. Cludts, S., Decaestecker, C., Mahillon, V., Chevalier, D., Kaltner, H., André, S., Remmelink, M., Leroy, X., Gabius, H.-J., Saussez, S. (2009) Galectin-8 up-regulation during hypopharyngeal and laryngeal tumor progression and comparison with galectin-1, -3 and -7. Anticancer Res. 29, 4933-4940.
11. Dagher, S. F., Wang, J. L., Patterson, R. J. (1995) Identification of galectin-3 as a factor in pre-mRNA splicing. Proc. Natl. Acad. Sci. USA 92, 1213-1217. <https://doi.org/10.1073/pnas.92.4.1213>
12. Davidson, P. J., Davis, M. J., Patterson, R. J., Ripoche, M.A., Poirier, F., Wang, J. L. (2002) Shuttling of galectin-3 between the nucleus and cytoplasm. Glycobiology 12, 329-337. <https://doi.org/10.1093/glycob/12.5.329>
13. Delacour, D., Gouyer, V., Zanetta, J. P., Drobecq, H., Leteurtre, E., Grard, G., Moreau-Hannedouche, O., Maes, E., Pons, A., André, S., Le Bivic, A., Gabius, H.-J., Manninen, A., Simons, K., Huet, G. (2005) Galectin-4 and sulfatides in apical membrane trafficking in enterocyte-like cells. J. Cell Biol. 169, 491-501. <https://doi.org/10.1083/jcb.200407073>
14. Díez-Revuelta, N., Velasco, S., André, S., Kaltner, H., Kübler, D., Gabius, H.-J., Abad-Rodriguez, J. (2010) Phosphorylation of adhesionand growth-regulatory human galectin-3 leads to the induction of axonal branching by local membrane L1 and ERM redistribution. J. Cell Sci. 123, 671-681. <https://doi.org/10.1242/jcs.058198>
15. Dvořánková, B., Lacina, L., Smetana Jr, K., Lensch, M., Manning, J. C., André, S., Gabius, H.-J. (2008) Human galectin-2: nuclear presence in vitro and its modulation by quiescence/stress factors. Histol. Histopathol. 23, 167-178.
16. Fedorova, E., Zink, D. (2008) Nuclear architecture and gene regulation. Biochim. Biophys. Acta 1783, 2174-2184. <https://doi.org/10.1016/j.bbamcr.2008.07.018>
17. Folle, G. A. (2008) Nuclear architecture, chromosome domains and genetic damage. Mutat. Res. 658, 172-183. <https://doi.org/10.1016/j.mrrev.2007.08.005>
18. Gabius, H.-J. (1987) Endogenous lectins in tumors and the immune system. Cancer Invest. 5, 39-46. <https://doi.org/10.3109/07357908709020305>
19. Gabius, H.-J. (2006) Cell surface glycans: the why and how of their functionality as biochemical signals in lectin-mediated information transfer. Crit. Rev. Immunol. 26, 43-80. <https://doi.org/10.1615/CritRevImmunol.v26.i1.30>
20. Gabius, H.-J. (2009a) Animal and human lectins. In Gabius, H.J. (ed.) The Sugar Code. Fundamentals of Glycosciences. Wiley-VCH, Weinheim, Germany, pp 317-328.
21. Gabius, H.-J. (ed.) (2009b) The Sugar Code. Fundamentals of Glycosciences. Wiley-VCH, Weinheim, Germany.
22. Gabius, H.-J., Brehler, R., Schauer, A., Cramer, F. (1986) Localization of endogenous lectins in normal human breast, benign breast lesions and mammary carcinomas. Virch. Arch. [Cell. Pathol.] 52, 107-115. <https://doi.org/10.1007/BF02889955>
23. Gabius, H.-J., Bodanowitz, S., Schauer, A. (1988) Endogenous sugar-binding proteins in human breast tissue and benign and malignant breast lesions. Cancer 61, 1125-1131. <https://doi.org/10.1002/1097-0142(19880315)61:6<1125::AID-CNCR2820610612>3.0.CO;2-L>
24. Gabius, H.-J, Wosgien, B., Hendrys, M., Bardosi, A. (1991) Lectin localization in human nerve by biochemically defined lectin-binding glycoproteins, neoglycoprotein and lectin-specific antibody. Histochemistry 95, 269-277. <https://doi.org/10.1007/BF00744999>
25. Haudek, K. C., Patterson, R. J., Wang, J. L. (2010) SR proteins and galectins: what’s in a name? Glycobiology 20, 1199-1207. <https://doi.org/10.1093/glycob/cwq097>
26. Hudson, D. F., Marshall, K. M., Earnshaw, W. C. (2009) Condensin: architect of mitotic chromosomes. Chromosome Res. 17, 131-144. <https://doi.org/10.1007/s10577-008-9009-7>
27. Kaltner, H., Seyrek, K., Heck, A., Sinowatz, F., Gabius, H.-J. (2002) Galectin-1 and galectin-3 in fetal development of bovine respiratory and digestive tracts. Comparison of cell type-specific expression profiles and subcellular localization. Cell Tissue Res. 307, 35-46. <https://doi.org/10.1007/s004410100457>
28. Kitagawa, R. (2010) Key players in chromosome segregation in Caenorhabditis elegans. Front. Biosci. 14, 1529-1557.
29. Kopitz, J., von Reitzenstein, C., André, S., Kaltner, H., Uhl, J., Ehemann, V., Cantz, M., Gabius, H.-J. (2001) Negative regulation of neuroblastoma cell growth by carbohydratedependent surface binding of galectin-1 and functional divergence from galectin-3. J. Biol. Chem. 276, 35917-35923. <https://doi.org/10.1074/jbc.M105135200>
30. Kopitz, J., Bergmann, M., Gabius, H.-J. (2010) How adhesion/growth-regulatory galectins-1 and -3 attain cell specificity: case study defining their target on neuroblastoma cells (SK-N-MC) and marked affinity regulation by affecting microdomain organization of the membrane. IUBMB Life 62, 624-628. <https://doi.org/10.1002/iub.358>
31. Kübler, D., Hung, C.-W., Dam, T. K., Kopitz, J., André, S., Kaltner, H., Lohr, M., Manning, J. C., He, L., Wang, H., Middelberg, A., Brewer, C. F., Reed, J., Lehmann, W. D., Gabius, H.-J. (2008) Phosphorylated human galectin-3: facile large-scale preparation of active lectin and detection of structural changes by CD spectroscopy. Biochim. Biophys. Acta 1780, 716-722. <https://doi.org/10.1016/j.bbagen.2008.01.018>
32. Lacina, L., Smetana, K., Jr., Dvořánková, B., Pytlík, R., Kideryová, L., Kučerová, L., Plzáková, Z., Štork, J., Gabius, H.-J., André, S. (2007) Stromal fibroblasts from basal cell carcinoma affect phenotype of normal keratinocytes. Br. J. Dermatol. 156, 819-829. <https://doi.org/10.1111/j.1365-2133.2006.07728.x>
33. Laing, J. G., Wang, J. L. (1988) Identification of carbohydrate binding protein 35 in heterogeneous nuclear ribonucleoprotein complex. Biochemistry 27, 5329-5334. <https://doi.org/10.1021/bi00414a057>
34. Langbein, S., Brade, J., Badawi, J. K., Hatzinger, M., Kaltner, H., Lensch, M., Specht, K., André, S., Brinck, U., Alken, P., Gabius, H.-J. (2007) Gene-expression signature of adhesion/growth-regulatory tissue lectins (galectins) in transitional cell cancer and its prognostic relevance. Histopathology 51, 681-690. <https://doi.org/10.1111/j.1365-2559.2007.02852.x>
35. Lin, H. M., Pestell, R. G., Raz, A., Kim, H. R. (2002) Galectin-3 enhances cyclin D1 promoter activity through SP1 and a cAMP-responsive element in human breast epithelial cells. Oncogene 21, 8001-8010. <https://doi.org/10.1038/sj.onc.1205820>
36. Liu, Y.-H., D’Ambrosio, M., Liao, T.-D., Peng, H., Rhaleb, N.-E., Sharma, U. C., André, S., Gabius, H.-J., Carretero, O. A. (2009) N-Acetyl-seryl-aspartyl-lysyl-proline prevents cardiac remodeling and dysfunction induced by galectin-3, a mammalian adhesion/growth-regulatory lectin. Am. J. Physiol. Heart Circ. Physiol. 296, H404-H412. <https://doi.org/10.1152/ajpheart.00747.2008>
37. Lohr, M., Lensch, M., André, S., Kaltner, H., Siebert, H.-C., Smetana Jr., K., Sinowatz, F., Gabius, H.-J. (2007) Murine homodimeric adhesion/growth-regulatory galectins-1, -2 and -7: comparative profiling of gene/promoter sequences by database mining, of expression by RT-PCR/immunohistochemistry and of contact sites for carbohydrate ligands by computational chemistry. Folia Biol. (Praha) 53, 109-128.
38. Lohr, M., Kaltner, H., Lensch, M., André, S., Sinowatz, F., Gabius, H.-J. (2008) Cell-type-specific expression of murine multifunctional galectin-3 and its association with follicular atresia/luteolysis in contrast to pro-apoptotic galectins-1 and -7. Histochem. Cell Biol. 130, 567-581. <https://doi.org/10.1007/s00418-008-0465-0>
39. Misteli, T. (2005) Concepts in nuclear architecture. Bioessays 27, 477-487. <https://doi.org/10.1002/bies.20226>
40. Moisa, A., Fritz, P., Eck, A., Wehner, H.-D., Mürdter, T., Simon, W., Gabius, H.-J. (2007) Growth/adhesion-regulatory tissue lectin galectin-3: stromal presence but not cytoplasmic/nuclear expression of tumor cells as negative prognostic factor in breast cancer. Anticancer Res. 27, 2131-2140.
41. Nasrabadi, D., Larijani, M. R., Fatuj, A., Gourabi, H., Dizajn A. V., Baharvand, H., Salekdeh, G. H. (2010) Nuclear proteome analysis of monkey embryonic stem cells during differentiation. Stem Cell Rev. 6, 50–61. <https://doi.org/10.1007/s12015-009-9109-6>
42. Oegema, K., Hyman, A. A. (2006) Cell division. In WormBook ed. The C. elegans Research Community, WormBook, doi/10.1895/wormbook.1.72.1, http://www.wormbook.org. <https://doi.org/10.1895/wormbook.1.72.1>
43. Paron, I., Scaloni, A., Pines, A., Bachi, A., Liu, F. T., Puppin, C., Pandolfi, M., Ledda, L., Di Loreto, C., Damante, G., Tell, G. (2003) Nuclear localization of Galectin-3 in transformed thyroid cells: a role in transcriptional regulation. Biochem. Biophys. Res. Commun. 302, 545-553. <https://doi.org/10.1016/S0006-291X(03)00151-7>
44. Patterson, R. J., Wang, W., Wang, J. L. (2004) Understanding the biochemical activities of galectin-1 and galectin-3 in the nucleus. Glycoconjugate J. 19, 499-506. <https://doi.org/10.1023/B:GLYC.0000014079.87862.c7>
45. Plzák, J., Betka, J., Smetana Jr., K., Chovanec, M., Kaltner, H., André, S., Kodet, R., Gabius, H.-J. (2004) Galectin-3 – an emerging prognostic indicator in advanced head and neck carcinoma. Eur. J. Cancer 40, 2324-2330. <https://doi.org/10.1016/j.ejca.2004.06.025>
46. Purkrábková, T., Smetana Jr., K., Dvořánková, B., Holíková, Z., Böck, C., Lensch, M., André, S., Pytlík, R., Liu, F.-T., Klíma, J., Smetana, K., Motlík, J., Gabius, H.-J. (2003) New aspects of galectin functionality in nuclei of cultured bone marrow stromal and epidermal cells: biotinylated galectins as tool to detect specific binding sites. Biol. Cell 95, 535-545. <https://doi.org/10.1016/j.biolcel.2003.08.002>
47. Rotblat, B., Niv, H., André, S., Kaltner, H., Gabius, H.-J., Kloog, Y. (2004) Galectin-1(L11A) predicted from a computed galectin-1 farnesyl-binding pocket selectively inhibits Ras-GTP. Cancer Res. 64, 3112-3118. <https://doi.org/10.1158/0008-5472.CAN-04-0026>
48. Saal, I., Nagy, N., Lensch, M., Lohr, M., Manning, J. C., Decaestecker, C. André, S., Kiss, R., Salmon, I., Gabius, H.-J. (2005) Human galectin-2: expression profiling by RTPCR/immunohistochemistry and its introduction as histochemical tool for ligand localization. Histol. Histopathol. 20, 1191-1208.
49. Sanchez-Ruderisch, H., Fischer, C., Detjen, K. M., Welzel, M., Wimmel, A., Manning, J. C., André, S., Gabius, H.-J. (2010) Tumor suppressor p16INK4a: downregulation of galectin-3, an endogenous competitor of the pro-anoikis effector galectin-1, in a pancreatic carcinoma model. FEBS J. 277, 3552-3563. <https://doi.org/10.1111/j.1742-4658.2010.07764.x>
50. Saussez, S., Decaestecker, C., Lorfevre, F., Chevalier, D., Mortuaire, G., Kaltner, H., André, S., Toubeau, G., Gabius, H.-J., Leroy, X. (2008) Increased expression and altered intracellular distribution of adhesion/growth-regulatory lectins galectins-1 and -7 during tumour progression in hypopharyngeal and laryngeal squamous cell carcinomas. Histopathology 52, 483-493. <https://doi.org/10.1111/j.1365-2559.2008.02973.x>
51. Saussez, S., Decaestecker, C., Cludts, S., Ernoux, P., Chevalier, D., Smetana Jr., K., André, S., Leroy, X., Gabius, H.-J. (2009) Adhesion/growth-regulatory tissue lectin galectin-1 in relation to angiogenesis/lymphocyte infiltration and prognostic relevance of stromal up-regulation in laryngeal carcinomas. Anticancer Res. 29, 59-66.
52. Saussez, S., de Leval, L., Decaestecker, C., Sirtaine, N., Cludts, S., Duray, A., Chevalier, D., André, S., Gabius, H.J., Remmelink, M., Leroy, X. (2010) Galectin fingerprinting in Warthin’s tumors: lectin-based approach to trace its origin? Histol. Histopathol. 25, 541-550.
53. Shimura, T., Takenaka, Y., Tsutsumi, S., Hogan, V., Kikuchi, A., Raz, A. (2004) Galectin-3, a novel binding partner of β-catenin. Cancer Res. 64, 6363-6367. <https://doi.org/10.1158/0008-5472.CAN-04-1816>
54. Smetana Jr., K., Dvořánková, B., Chovanec, M., Bouček, J., Klíma, J., Motlík, J., Lensch, M., Kaltner, H., André, S., Gabius, H.-J. (2006) Nuclear presence of adhesion-/growthregulatory galectins in normal/malignant cells of squamous epithelial origin. Histochem. Cell Biol. 125, 171-182. <https://doi.org/10.1007/s00418-005-0074-0>
55. Smetana, K., Jirásková, I., Otevřelová, P., Kalousek, I. (2008) The RNA content of nucleolar bodies is related to their size – a cytochemical study on human monocytes and lymphocytes in blood smears and blood cytospins. Folia Biol. (Praha) 54, 130-133. <https://doi.org/10.14712/fb2008054040130>
56. Solís, D., Maté, M. J., Lohr, M., Ribeiro, J. P., López-Merino, L., André, S., Buzamet, E., Cañada, F. J., Kaltner, H., Lensch, M., Ruiz, F. M., Haroske, G., Wollina, U., Kloor, M., Kopitz, J., Sáiz, J. L., Menéndez, M., Jiménez-Barbero, J., Romero, A., Gabius, H.-J. (2010) N-domain of human adhesion/growth-regulatory galectin-9: preference for distinct conformers and non-sialylated N-glycans and detection of ligand-induced structural changes in crystal and solution. Int. J. Biochem. Cell Biol. 42, 1019-1029. <https://doi.org/10.1016/j.biocel.2010.03.007>
57. Szabo, P., Dam, T., K., Smetana Jr., K., Dvořánková, B., Kübler, D., Brewer, C., F., Gabius, H.-J. (2009) Phosphorylated human lectin galectin-3: analysis of ligand binding by histochemical monitoring of normal/malignant squamous epithelia and by isothermal titration kalorimetry. Anat. Histol. Embryol. 38, 67-75. <https://doi.org/10.1111/j.1439-0264.2008.00899.x>
58. Villalobo, A., Nogales-Gonzalés, A., Gabius, H.-J. (2006) A guide to signaling pathways connecting protein-glycan interaction with the emerging versatile effector functionality of mammalian lectins. Trends Glycosci. Glycotechnol. 18, 1-37. <https://doi.org/10.4052/tigg.18.1>
59. Vyakarnam, A., Lenneman, A. J., Lakkides, K. M., Patterson, R. J., Wang J. L. (1998) A comparative nuclear localization study of galectin-1 with other splicing components. Exp. Cell Res. 242, 419-428. <https://doi.org/10.1006/excr.1998.4111>
60. Wang, J., Lu, Z. H., Gabius, H.-J., Rohowsky-Kochan, C., Ledeen, R. W., Wu, G. (2009) Cross-linking of GM1 ganglioside by galectin-1 mediates regulatory T cell activity involving TRPC5 channel activation: possible role in suppressing experimental autoimmune encephalomyelitis. J. Immunol. 182, 4036-4045. <https://doi.org/10.4049/jimmunol.0802981>
61. Wang, J. L, Gray, R. M., Haudek, K.C., Patterson, R. J. (2004) Nucleocytoplasmic lectins. Biochim. Biophys. Acta 1673, 75-93. <https://doi.org/10.1016/j.bbagen.2004.03.013>
62. Wang, L., Inohara, H., Pienta, K. J., Raz, A. (1995) Galectin-3 is a nuclear matrix protein which binds RNA. Biochem. Biophys. Res. Commun. 217, 292-303. <https://doi.org/10.1006/bbrc.1995.2777>
63. Woodcock, L. W. (2006) Chromatin architecture. Curr. Opin. Struct. Biol. 16, 213-220. <https://doi.org/10.1016/j.sbi.2006.02.005>
front cover

ISSN 0015-5500 (Print) ISSN 2533-7602 (Online)

Open access journal

Submissions

Archive