Fol. Biol. 2012, 58, 135-143

https://doi.org/10.14712/fb2012058040135

Early Stages of Trachea Healing Process: (Immuno/Lectin) Histochemical Monitoring of Selected Markers and Adhesion/ Growth-Regulatory Endogenous Lectins

T. Grendel1, J. Sokolský1, A. Vaščáková1, V. Hudák1, M. Chovanec2,3, F. Sabol4, S. André5, H. Kaltner5, H.-J. Gabius5, M. Frankovičová6, P. Lenčeš1, J. Betka2, K. Smetana, Jr.3, Peter Gál7,8,9

11st Department of Anesthesiology and Intensive Medicine, Pavol Jozef Šafárik University and Louis Pasteur University Hospital, Košice, Slovak Republic
2Clinic of Otorhinolaryngology and Head and Neck Surgery, First Faculty of Medicine, Charles University in Prague and University Hospital Motol, Prague, Czech Republic
3Institute of Anatomy, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
4Clinic of Heart Surgery, East-Slovak Institute of Cardiovascular Diseases, Košice, Slovak Republic
5Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians University, Munich, Germany
6Clinic of Vascular Surgery, East-Slovak Institute of Cardiovascular Diseases, Košice, Slovak Republic
7Department for Biomedical Research, East-Slovak Institute of Cardiovascular Diseases, Košice, Slovak Republic
8Department of Pathological Anatomy, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic
9Institute of Histology and Embryology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic

Received January 2012
Accepted February 2012

Tracheotomy may be associated with numerous acute and chronic complications including extensive formation of granulation tissue. The emerging functional versatility of the adhesion/growthregulatory galectins prompted us to perform a histochemical study of wound healing using rat trachea as model. By using non-cross-reactive antibodies and the labelled tissue lectins we addressed the issue of the presence and regulation of galectin reactivity during trachea wound healing. Beside localization of high-molecular-weight keratin, wide-spectrum cytokeratin, keratins 10 and 14, α-smooth muscle actin, vimentin, fibronectin, and Sox-2, galectins -1, -2, and -3 and their reactivity profiles were measured in frozen sections of wounded and control trachea specimens 7, 14, and 28 days after trauma. A clear trend for decreased galectin-1 presence and increased reactivity for galectin-1 was revealed from day 7 to day 28. Sox-2-positive cells were present after seven days and found in the wound bed. Interestingly, several similarities were observed in comparison to skin wound healing including regulation of galectin-1 parameters.

Funding

This study was supported by the EC research program GlycoHIT (contract Id 260600), Slovak Grant Agency of Ministry of Education (VEGA No. 1/1095/11), and Charles University in Prague (project for support of specific university student research).

References

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