Paraoxonase-1 (PON1) Status in Pancreatic Cancer: Relation to Clinical Parameters

Vecka, Marek; Jáchymová, M.; Vávrová, L.; Kodydková, J.; Macášek, J.; Urbánek, M.; Krechler, T.; Slabý, A.; Dušková, J.; Muravská, A.; Žák, A.

doi:10.14712/fb2012058060231

Folia Biologica > Archive > 2012, Vol. 58 > Issue 6 > Paraoxonase-1 (PON1) Status in…

Fol. Biol. 2012, 58, 231-237

https://doi.org/10.14712/fb2012058060231

Paraoxonase-1 (PON1) Status in Pancreatic Cancer: Relation to Clinical Parameters

Marek Vecka¹, M. Jáchymová², L. Vávrová¹, J. Kodydková¹, J. Macášek¹, M. Urbánek¹, T. Krechler¹, A. Slabý¹, J. Dušková³, A. Muravská², A. Žák¹

¹4th Department of Internal Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Czech Republic
²Institute for Clinical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Czech Republic
³Institute of Pathology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Czech Republic

Received June 2012
Accepted June 2012

Human paraoxonase 1 (PON1) has been shown to decrease the level of systemic oxidative stress, which is thought to contribute to cancer development. The aim of this study was to examine the interrelationships between PON1 status and some clinical characteristics in patients with pancreatic cancer (PC). A group of 73 consecutive patients with PC (stage II–IV) and 73 control subjects were examined. Laboratory studies included five polymorphisms of the PON1 gene (L55M, Q192R, -108C/T, -126C/T, and -162A/G), PON1 arylesterase (PON1-A) and lactonase (PON1-L) activities, as well as some markers of protein metabolism, insulin resistance, and oxidative stress. In comparison with the control group, no difference in the distribution of the PON1 polymorphisms was found in cancer patients, both arylesterase and lactonase activities being significantly lower (-33, -47 %, respectively, both P < 0.001). There was neither statistically significant association of PON1 polymorphisms with tumour stages nor with diabetes mellitus connected with PC. The genotype distribution of L55M and -108C/T differed only in a subgroup of patients presenting clinically relevant malnutrition (χ² = 6.50, 6.25, respectively, both P < 0.05). In the PC group, PON1-A and PON1-L activities correlated with Nutritional Risk Index (r = 0.351, 0.409, respectively, both P < 0.01), PON1-L with mid-arm muscle circumference (r = 0.328, P < 0.05), and PON1-A and PON1-L with serum albumin (r = 0.352, 0.391 respectively, both < 0.01). Our results suggest that PON1 plays an important role in PC, especially in cancer-associated malnutrition.