Fol. Biol. 2013, 59, 47-50
Validation of RNA Extraction Procedures Focused on Micro RNA Expression Analysis
The sampling procedure is a crucial step in every kind of experiment. This is also true in gene expression profiling experiments, where high quality and sufficient quantity of extracted RNA plays a significant role in the experimental outcome. We have compared five different RNA extraction protocols from peripheral blood/PBMCs with the aim to define the most suitable method for the miRNA expression profiling experiments. Convincing results in terms of highest quantity and quality were obtained by the TRIzol-chloroform extraction method. The total RNA obtained using this method contained the highest portion of good-quality miRNA molecules, which was also confirmed by gene-specific real-time PCR experiments.
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Funding
This work was supported by the Internal Grant Agency of the Ministry of Health of the Czech Republic (MZČR NT 12452-4) and by the project of the Ministry of Health of the Czech Republic for conceptual development of research organization 023728 (Institute of Rheumatology).
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Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.
