Association of Obesity Susceptibility Gene Variants with Metabolic Syndrome and Related Traits in 1,443 Czech Adolescents

Dušátková, Lenka; Zamrazilová, H.; Sedláčková, B.; Včelák, J.; Hlavatý, P.; Aldhoon Hainerová, I.; Korenková, V.; Bradnová, O.; Bendlová, B.; Kunešová, M.; Hainer, V.

doi:10.14712/fb2013059030123

Folia Biologica > Archive > 2013, Vol. 59 > Issue 3 > Association of Obesity Susceptibility…

Fol. Biol. 2013, 59, 123-133

https://doi.org/10.14712/fb2013059030123

Association of Obesity Susceptibility Gene Variants with Metabolic Syndrome and Related Traits in 1,443 Czech Adolescents

Lenka Dušátková^1,2, H. Zamrazilová², B. Sedláčková^2,3, J. Včelák⁴, P. Hlavatý², I. Aldhoon Hainerová^2,5, V. Korenková⁶, O. Bradnová^4,7, B. Bendlová⁴, M. Kunešová², V. Hainer²

¹Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Prague, Czech Republic
²Obesity Management Centre, Institute of Endocrinology, Prague, Czech Republic
³Department of Biochemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic
⁴Department of Molecular Endocrinology, Institute of Endocrinology, Prague, Czech Republic
⁵Department of Paediatrics and Centre for Research of Diabetes, Metabolism and Nutrition, Third Faculty of Medicine, Charles University in Prague, Czech Republic
⁶Laboratory of Gene Expression, Institute of Biotechnology AS CR, v. v. i., Prague, Czech Republic
⁷Department of Anthropology and Human Genetics, Faculty of Science, Charles University in Prague, Prague, Czech Republic

Received January 2013
Accepted February 2013

Genome-wide association studies have revealed several gene variants associated with obesity; however, only a few studies have further investigated their association with metabolic syndrome. We performed a study of eleven variants in/near genes TMEM18, SH2B1, KCTD15, PCSK1, BDNF, SEC16B, MC4R, and FTO in Czech adolescents and analysed their association with obesity, metabolic syndrome and related traits. Genotyping was performed in 1,443 adolescents aged 13.0–17.9 years. Anthropometric parameters, biochemical parameters and blood pressure were assessed. Metabolic syndrome was defined according to the International Diabetes Federation. The FTO rs9939609 variant was associated with overweight/obesity (OR 1.40, 95% CI 1.21–1.63, P < 0.001). The minor allele of TMEM18 rs7561317 was related to underweight (OR 1.78, 95% CI 1.14–2.79, P = 0.015). BDNF rs925946 and MC4R rs17782313 were associated with metabolic syndrome (OR 1.53, 95% CI 1.14–2.04, P = 0.005; 1.51, 95% CI 1.12–2.04, P = 0.009). The PCSK1 rs6235 variant was negatively related to increased blood glucose (OR 0.69, 95% CI 0.49–0.97, P = 0.040). In conclusion, the FTO variant was associated with overweight/obesity in Czech adolescents. Moreover, MC4R and BDNF variants increased the risk of metabolic syndrome, probably through their effect on abdominal obesity. The PCSK1 variant may have a protective role in the development of type 2 diabetes.

Keywords

single-nucleotide polymorphism, FTO gene, obesity, metabolic syndrome, adolescence.

Funding

This study was supported by GAUK 370911 of the Charles University Grant Agency, NT/13792 of the Internal Grant Agency of the Ministry of Health of the Czech Republic, 7F08077 of the Ministry of Education, Youth and Sports of the Czech Republic, grant of the Danone Institute and CZ0123 from Norway through the Norwegian Financial Mechanisms.