Fol. Biol. 2013, 59, 134-137
Comparative Analysis of IL-8 and CXCL-1 Production by Normal and Cancer Stromal Fibroblasts
It has been shown that fibroblasts within the stroma of malignant tumours can affect the tumour’s biological character, influencing such properties as local aggressiveness and metastasis potential. This influence is asserted via paracrine secretion of multiple cell factors, including chemokines. This study demonstrates that both normal keratinocytes and cancer cells can stimulate the secretion of chemokines IL-8 and CXCL-1 from normal dermal fibroblasts and stromal fibroblasts from squamous cell carcinoma. The effect of epithelia on normal fibroblasts leads to a transient secretory change, in contrast to stromal fibroblasts which generate a more prolonged one. This observation demonstrates that stimulated expression of both IL-8 and CXCL-1 is not specific to cancer, supporting the hypothesis that similar mechanisms exist between wound healing and oncogenesis. It also shows that stromal fibroblasts isolated from a tumour have significantly different features from normal fibroblasts.
Keywords
chemokine, cancer-associated fibroblasts, cancer microenvironment, wound healing, cancer stroma.
Funding
This study was supported by Charles University in Prague, projects Nos. SVV 264510, PRVOUK 27-1 and UNCE 204013, and by the Grant Agency of the Ministry of Health of the Czech Republic, grant No. NT/13488.
References
Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.
