Effects of Depsidones from <i>Hypogymnia physodes</i> on HeLa Cell Viability and Growth

Stojanović, I. Ž.; Najman, S.; Jovanović, O.; Petrović, G.; Najdanović, J.; Vasiljević, P.; Šmelcerović, Andrija

doi:10.14712/fb2014060020089

Folia Biologica > Archive > 2014, Vol. 60 > Issue 2 > Effects of Depsidones from Hypogymnia…

Fol. Biol. 2014, 60, 89-94

https://doi.org/10.14712/fb2014060020089

Effects of Depsidones from Hypogymnia physodes on HeLa Cell Viability and Growth

I. Ž. Stojanović¹, S. Najman², O. Jovanović³, G. Petrović³, J. Najdanović², P. Vasiljević⁴, Andrija Šmelcerović¹

¹Department of Pharmacy, Faculty of Science and Mathematics, University of Nis, Nis, Serbia
²Institute of Biology and Human Genetics, Faculty of Medicine, University of Nis, Nis, Serbia
³Department of Chemistry, Faculty of Science and Mathematics, University of Nis, Nis, Serbia
⁴Department of Biology and Ecology, Faculty of Science and Mathematics, University of Nis, Nis, Serbia

Received November 2013
Accepted February 2014

The anti-proliferative activitiy of Hypogymnia physodes methanol extracts (ME) and its main constituents, physodalic acid (P1), physodic acid (P2), and 3-hydroxy physodic acid (P3), was tested on human cancer HeLa cell lines. Three lichen depsidones, P1, P2 and P3, were isolated from H. physodes ME using column chromatography and their structures were determined by UV, ESI TOF MS, ¹H and ¹³C NMR. The content of P1, P2 and P3 in ME was determined using reversed-phase highperformance liquid chromatography with photodiode array detection. P1–3 represented even 70 % of the studied extract. The HeLa cells were incubated during 24 and 72 h in the presence of ME and depsidones P1, P2 and P3, at concentrations of 10–1000 μg/ml. Compounds P2 and P3 showed higher activity than compound P1. Half maximal inhibitory concentrations (IC₅₀, μg/ml) of P1, P2, P3 and ME for 24-h incubation were 964, 171, 97 and 254 μg/ml, respectively, while for 72-h incubation they were 283, 66, 63 and 68 μg/ml. As far as we know, this is the first report on the effect of H. physodes ME and their depsidones on HeLa cells.

Keywords

Hypogymnia physodes, anti-proliferative activity, HeLa cells, physodic acid, 3-hydroxyphysodic acid, physodalic acid.

Funding

The authors acknowledge the financial support of the Ministry of Education, Science and Technological Development of Serbia (Grants Nos. 172044 and 41017 WP4).