Differential Expression of Tripartite Motif-Containing Family in Normal Human Dermal Fibroblasts in Response to Porcine Endogenous Retrovirus Infection

Kimsa, Malgorzata W.; Strzalka-Mrozik, B.; Kimsa, M. C.; Mazurek, U.; Kruszniewska-Rajs, C.; Gola, J.; Adamska, J.; Twardoch, M.

doi:10.14712/fb2014060030144

Fol. Biol. 2014, 60, 144-151

https://doi.org/10.14712/fb2014060030144

Differential Expression of Tripartite Motif-Containing Family in Normal Human Dermal Fibroblasts in Response to Porcine Endogenous Retrovirus Infection

Malgorzata W. Kimsa¹, B. Strzalka-Mrozik¹, M. C. Kimsa², U. Mazurek¹, C. Kruszniewska-Rajs¹, J. Gola¹, J. Adamska¹, M. Twardoch¹

¹Department of Molecular Biology, Medical University of Silesia, Sosnowiec, Poland
²Department of Food and Nutrition, Medical University of Silesia, Sosnowiec, Poland

Received October 2013
Accepted March 2014

Antiretroviral restriction factors may play an essential role in the safety of xenotransplantation. Therefore, the present study focused on investigation of the changes in the tripartite motif-containing family (TRIM) gene expression in normal human dermal fibroblasts with and without lipopolysaccharide stimulation in response to porcine endogenous retrovirus infection. Analysis of the expression profile of TRIMs was performed using oligonucleotide microarrays and QRT-PCR. Nine (TRIM1, TRIM2, TRIM5, TRIM14, TRIM16, TRIM18, TRIM22, TRIM27 and TRIM31) statistically significantly differentially expressed genes were found (P < 0.05, one-way ANOVA). In conclusion, comprehensive analysis of retroviral restriction factor gene expression in human dermal fibroblasts before and after porcine endogenous retrovirus infection with and without LPS stimulation may suggest association of the selected TRIMs with antiretroviral activity.