Complementarity between Microarray and Immunoblot for the Comparative Evaluation of IgE Repertoire of French and Italian Cypress Pollen Allergic Patients

Shahali, Y.; Nicaise, P.; Brázdová, A.; Charpin, D.; Scala, E.; Mari, A.; Sutra, J. P.; Chollet-Martin, S.; Sēnēchal, H.; Poncet, Pascal

doi:10.14712/fb2014060040192

Folia Biologica > Archive > 2014, Vol. 60 > Issue 4 > Complementarity between Microarray and…

Fol. Biol. 2014, 60, 192-201

https://doi.org/10.14712/fb2014060040192

Complementarity between Microarray and Immunoblot for the Comparative Evaluation of IgE Repertoire of French and Italian Cypress Pollen Allergic Patients

Y. Shahali^1,2, P. Nicaise³, A. Brázdová^4,5, D. Charpin⁶, E. Scala⁷, A. Mari⁸, J. P. Sutra¹, S. Chollet-Martin^3,9, H. Sēnēchal¹, Pascal Poncet^1,10

¹Armand Trousseau Hospital, Biochemistry and Molecular Biology Department, Allergy & Environment Group, Paris, France
²PERSIFLORE®, Le PlessisPâté, France
³Bichat-Claude Bernard Hospital, Auto-immunity & Hypersensitivity Department, Paris, France
⁴INSERM UMR872, Cordelier Research Centre, Paris, France
⁵Institute of Chemical Technology, Department of Biochemistry and Microbiology, Prague, Czech Republic
⁶North Hospital, Pneumo-Allergology Department, Marseille Cedex 20, France
⁷IDI-IRCCS, Experimental Allergology Department, Rome, Italy
⁸CAAM, Latina, Italy
⁹INSERM UMR 996, Paris-South University, Immunopathology Department, Châtenay-Malabry, France
¹⁰Pasteur Institute, Infections & Epidemiology Department, Paris, France

Received January 2014
Accepted April 2014

Cypress pollen represents the primary cause of respiratory allergies in Mediterranean areas. Patients allergic to Cupressus sempervirens pollen (Cups) (CPA) can be discriminated on the basis of the immunoglobulin E (IgE) binding to a basic 14 kDa protein (BP14) or to high-molecular-weight (HMW) glycoproteins only. Specific IgE repertoires of two differentially exposed CPA cohorts, French and Italian, were investigated using an IgE microarray system (some known major allergens from several allergenic sources) and individual IgE immunoblotting (IB) of whole Cups pollen extract separated by SDS-PAGE (all allergens from one allergenic source: cypress pollen). The prevalence of sensitization to BP14 was higher in French (37 %) than in Italian patients (17 %) and major differences were observed in IgE reactivities to lipid transfer proteins (LTPs). Thirty percent of the Italian CPA (4 % in the French group) had specific IgE against the Parietaria pollen LTP, independently of IB subgroups. Regarding peach LTP sensitization, all Pru p 3+ Italian CPA (10 %) were in the HMW+ subgroup, while Pru p 3+ French CPA (20 %) were all included in the BP14+ subgroup. BP14 sensitization is likely a marker of Cups exposure and is, in French CPA, significantly correlated to Pru p 3 sensitization. The IgE immunoblot and microarray are complementary tools that highlight differences in the subtle sensitization profile between groups of patients in comparative studies.