Fol. Biol. 2014, 60, 213-219
SKOV-3 and Me45 Cell Response to Cisplatin-Based Chemotherapy: an in Vitro Study
We studied malignant melanoma cell line Me45 and human ovarian carcinoma cell line SKOV-3 (resistant to cisplatin, adriamycin and diphtheria toxin), assessing their expression level of p53, HSP70 and glutathione S-transferase GST-π before and after chemotherapy with cisplatin. These proteins may be responsible for the occurrence of chemoresistance in cancer patients. To assess protein expression we used the immunocytochemical Avidin-Biotin-peroxidase Complex (ABC) method. Before application of chemotherapy, proteins p53, HSP70 and GST-π were present in 100 % of the examined melanoma cells. After the treatment, the intensity of the immunocytochemical reaction for p53 increased, whereas the intensity of immunocytochemical staining for HSP70 and GST-π decreased. In SKOV-3 cells, p53 and HSP70 were present in 100 % of the examined cells both prior to chemotherapy and after it. However, the intensity of the immunocytochemical reaction for p53 decreased, while that of HSP70 increased. As regards GST-π, only 5 % of all examined SKOV-3 cells revealed its expression before chemotherapy. Incubation with cisplatin caused an elevation in the number of ovarian cancer cells expressing GST-π up to 50 %. Moreover, the intensity of the immunocytochemical reaction for GST-π significantly increased.
Keywords
ovarian cancer, melanoma, cisplatin, heat-shock proteins, glutathione S-transferase π, p53.
Funding
This work was supported by a grant for Young Scientists sponsored by Statutory Funds of Wroclaw Medical University supported by the Polish Ministry of Science and Higher Education, grants Nos. 131 PBmn and ST 648.
References
Copyright
This is an open-access article distributed under the terms of the Creative Commons Attribution License.
