Anthocyanin-Rich Diet in Chemically Induced Colitis in Mice

Janšáková, Katarína; Bábíčková, J.; Filová, B.; Lengyelová, E.; Havrlentová, M.; Kraic, J.; Celec, P.; Tóthová, Ľ.

doi:10.14712/fb2015061030104

Folia Biologica > Archive > 2015, Vol. 61 > Issue 3 > Anthocyanin-Rich Diet in Chemically…

Fol. Biol. 2015, 61, 104-109

https://doi.org/10.14712/fb2015061030104

Anthocyanin-Rich Diet in Chemically Induced Colitis in Mice

Katarína Janšáková¹, J. Bábíčková^1,2, B. Filová³, E. Lengyelová¹, M. Havrlentová^4,5, J. Kraic^4,5, P. Celec^1,2,6,7, Ľ. Tóthová^1,2

¹Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia
²Center for Molecular Medicine, Slovak Academy of Sciences, Bratislava, Slovakia
³Institute of Medical Physics, Biophysics, Informatics and Telemedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia
⁴Department of Biotechnologies, Faculty of Natural Sciences, University of SS. Cyril and Methodius, Trnava, Slovakia
⁵National Agricultural and Food Centre – Research Institute of Plant Production, Piešťany, Slovakia
⁶Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia
⁷Institute of Pathophysiology, Faculty of Medicine, Comenius University, Bratislava, Slovakia

Received February 2015
Accepted April 2015

The aetiology of inflammatory bowel diseases is unclear, but oxidative stress plays a key role in the pathogenesis. Anthocyanins – plant polyphenols – were shown to have antioxidant and anti-inflammatory properties. The aim of this study was to investigate the potential protective effects of anthocyanins on the oxidative status in mice with chemically induced colitis. Adult male mice were randomly divided into a control group drinking tap water and a colitis group drinking 1% dextran sulphate sodium solution. Animals had ad libitum access to a control wheat-based diet or food based on wheat producing anthocyanins. Bodyweight and stool consistency were monitored daily for 14 days. At the end of the experiment, colon length was measured and tissue samples were collected for the assessment of histology and oxidative status. Mice with colitis had lower body weight, higher stool score and shorter colon than control mice. Anthocyanins had neither an effect on stool consistency, nor on bodyweight loss and colon length. In the colon, liver and plasma, analysis of oxidative stress markers and antioxidant status revealed no significant differences between the groups. Food made from wheat producing anthocyanins did not protect mice from the consequences of chemically induced colitis. The measured biomarkers do not confirm the role of oxidative stress in this model of colitis. Further optimization of the anthocyanin- rich food might be needed before further experiments are conducted.

Keywords

flavonoids, protein oxidation, inflammatory bowel disease, oxidative stress.

Funding

This work is a result of the implementation of the project (ITMS: 26240220071) supported by the Research & Development Operational Programme funded by the ERDF, and by Comenius University Grant UK/452/2014.